Design of ionic liquid-based microwave-assisted extraction of flavonoids from Cyclea barbata Miers. And its lipoxygenase inhibitory activity

Islamudin Ahmad, Berna Elya, Yayuk Isneyni Ismail, Arikadia Noviani, Hadi Kuncoro, Maryono, Neneng Siti Silfi Ambarwati

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Green grass jelly (Cyclea barbata Miers.) is a plant from Indonesia that is believed to have anti-inflammatory activity. This study aims to find the optimum condition in grass jelly extraction using the ionic liquid-microwave assisted extraction toward total flavonoid content (TFC) and lipoxygenase activity (LIA). The experimental design was performed using the parameters variable including extraction time, liquid-solid ratio, and ionic liquids concentration to obtain the optimum condition. The optimization analysis used response surface methodology (RSM) with Box-Behnken design (17 trials) to obtain a predictive model with TFC and LIA as a response surface value. In the present study, the optimum condition was suggested by RSM analysis with parameter variables, including extraction time of 17 minutes, 1-butyl-3-methylimidazolium bromide ([BMIM]Br) concentration of 1.76 mol/l, and the liquid-solid ratio of 38.21 ml/g. The equation of regression quadratic model was obtained to predict TFC and LIA as follows: TFC = 2.43A + 2.43B + 1.42C + 0.33AB-3.20AC-0.46BC-4.90A2-3.10B2-3.10C2 + 28.32 with R2 = 0.8336 and LIA = 0.066A + 8.22B + 0.97C + 2.47AB-5.86AC + 1.96BC-9.99A2-13.75B2-13.11C2 + 63.53 with R2 = 0.9207, respectively.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Applied Pharmaceutical Science
Volume10
Issue number4
DOIs
Publication statusPublished - 2020

Keywords

  • Cyclea barbata miers.
  • Ionic liquid-microwave assisted extraction
  • Lipoxygenase activity inhibition
  • Response surface methodology
  • Total flavonoid content

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