This research describes designsand calibration of drum collector and Analog-to-Digital Converters (ADC) of high voltage for nano-fiber electrospinning based on microcontroller systems. Electrospinning is commonly used to make nanofibers electrostatically which then produces nanofiber polymers with a diameter in the nanometer scale from a high voltage polymer solution. Compared to conventional technology, electrospinning is more versatile and efficient technique. However, several important parameters must be considered in solutions, control processes, and the environment. High voltage and collector instrument are required for applying electrospinning technique. Collectors have a significant effect on the productivity and the arrangement of nanofibers and the final structure. The rotation speed or RPM of the drum collector must be controlled to obtain the optimal nanofibrous membranes. Voltage control is also an important factor in this electrospinning technique, therefore it is necessary to control the applied high voltage. The microcontroller used in this system is Arduino nano. The hardware in this system consists of A4988 motor driver, high voltage, drum collector. Communication between the microcontroller and ADC uses the Serial Peripheral Interface (SPI). Serial communication with baud rate of 9600 was used for communication between the microcontroller and PC. The calibration transfer function of drum collector is y = 0.0048 + 0.0015 with R square is 0.999. The calibration transfer function of ADC is y = 3.10e-7 - 0.0003 with R square = 1. ADC calibration values used have also been successfully carried out with error of 0.347%.