Pulse Electromagnetic Therapy (PEMFt) or better known as PEMF is one of the modalities approved by the Food and Drug Administration (FDA) to assist the healing process of non union bone fractures (failed connect). Currently PEMF equipment sold at a price that is not cheap, has a varying physical parameters so that in its use produce various effectiveness and sometimes contraindicated. In this research have been done design, manufacture and test prototype of PEMF tool using Arduino and Labview. The purpose of this research is to obtain PEMF tool which can be used in bioloectromagnetic research. The research was conducted in three stages, namely design and hardware design, design and software design, and the third step is PEMF testing for bone fracture research using mouse rats. In the first stage is made Helmholtz coil with a diameter of 40 cm, the number of windings 500, using 0.7 mm copper wire. The magnetic field characterization generated using Gaussmeter IDR 324 on the x axis, at a voltage of 18 V and a current of 0.28 A, produces a homogeneous magnetic field of 0.4 mT. In the second stage, the programming of PEMF tools was made using Labview and Arduino Uno micro controller. The controlled PEMF physical parameters are waveform, frequency, duty cycle, burst time and exposure time. Interface creation and data processing is done using LabVIEW program. In the third phase, a prototype PEMF test was made for a study of experimental animals of Spraque Dawley mice for 28 days.