Delivery of a heterologous antigen by a registered Salmonella vaccine (STM1)

Endang Winiati, Kuo Ching Sheng, Theodora Fifis, Anita Gamvrellis, Magdalena Plebanski, Peter J. Coloe, Peter M. Smooker

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

STM1 is an aro A- attenuated mutant of Salmonella enterica serovar Typhimurium, and is a well-characterised vaccine strain available to the livestock industry for the prevention of salmonellosis in chickens. This strain has potential for heterologous antigen delivery, and here we show that the strain can be used to deliver a model antigen, ovalbumin, to immune cells in vitro and in vivo. Two plasmid constructs expressing the ovalbumin gene were utilised, one of which uses a prokaryotic promoter and the other the CMV promoter (DNA vaccine). In vitro, STM1 carrying ovalbumin-encoding plasmids was able to invade dendritic cells and stimulate a CD8+ cell line specific for the dominant ovalbumin epitope, SIINFEKL. In vivo, spleen cells were responsive to SIINFEKL after vaccination of mice with ovalbumin-encoding plasmids in STM1, and finally, humoral responses, including IgA, were induced after vaccination.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalFEMS Microbiology Letters
Volume227
Issue number2
DOIs
Publication statusPublished - 24 Oct 2003

Keywords

  • Antigen presentation
  • Dendritic cell
  • Immune response
  • Salmonella

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