Decellularization of liver cubes using multiple site syringe injection for generating native liver scaffold: Preliminary report

Radiana D. Antarianto, A. A.Ayu Asri Prima Dewi, Adrian Pragiwaksana, Jeanne Adiwinata Pawitan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Scaffold is one of the important parts of tissue engineering. Liver scaffolds are obtained by decellularization of native liver. Frequently used decellularization methods include immersion or peristaltic pump perfusion techniques. Immersion technique requires longer duration and risk of tissue decay in the tissue core. Limited laboratory facilities have a peristaltic pump, thus limiting the usage of perfusion decellularization techniques. Therefore, the aim of this study was to develop a technique of simple liver decellularization with multiple site syringe injection. Material and methods: Isolated rat livers were freeze-thawed and cut into cubical form with a size of 1.5 cm × 1.5 cm × 0.7 cm up to 1 cm. The liver cubes were incubated in EGTA solution for 30 minutes and followed by multiple site syringe injection with increasing SDS concentration (0.1 %, 0.25%, 0.5%, 0.75%, and 1%) until appeared transparent (± 6 hours). The decellularized liver cubes were processed histologically and stained with hematoxylin-eosin. Result and discussion: Decellularized liver cubes using multiple site syringe injection compared to undecellularized liver showed removal of cells and structurally intact extracellular matrix as observed from histological specimens. Conclusions: Multiple site syringe injection is a simple technique to decellularized liver cubes for preparation of native liver scaffold for tissue engineering.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
CountryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • biological liver scaffold
  • liver decellularization
  • liver tissue engineering

Fingerprint Dive into the research topics of 'Decellularization of liver cubes using multiple site syringe injection for generating native liver scaffold: Preliminary report'. Together they form a unique fingerprint.

  • Cite this

    Antarianto, R. D., Dewi, A. A. A. A. P., Pragiwaksana, A., & Pawitan, J. A. (2019). Decellularization of liver cubes using multiple site syringe injection for generating native liver scaffold: Preliminary report. In K. Lischer, T. Abuzairi, S. F. Rahman, & M. Gozan (Eds.), 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019 [040005] (AIP Conference Proceedings; Vol. 2193). American Institute of Physics Inc.. https://doi.org/10.1063/1.5139367