TY - GEN
T1 - Decellularization of liver cubes using multiple site syringe injection for generating native liver scaffold
T2 - 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
AU - Antarianto, Radiana D.
AU - Dewi, A. A.Ayu Asri Prima
AU - Pragiwaksana, Adrian
AU - Pawitan, Jeanne Adiwinata
N1 - Funding Information:
This work is funded by Master Thesis Research Grant from the Ministry of Higher Education and Technology Republic of Indonesia year 2019 with contract number: NKB-1884/UN2.R3.1/HKP.05.00/2019. The authors acknowledged the work by Wildan Mubarok, M.Si as the research assistant at Stem cell and tissue engineering research cluster IMERI UI who performed validation of the liver cubes preparation.
Funding Information:
1Department of Histology, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia 2Stem Cell and Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia 3Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia 4Department of Histology, Faculty of Medicine, Warmadewa University, Jl. Terompong 24 Tanjung Bungkak, Denpasar, Bali, Indonesia 5Integrated Stem Cell Technology Service Unit, Cipto Mangunkusumo National General Hospital, Jl. Diponegoro No.71, Salemba, Jakarta, Indonesia
Publisher Copyright:
© 2019 Author(s).
PY - 2019/12/10
Y1 - 2019/12/10
N2 - Scaffold is one of the important parts of tissue engineering. Liver scaffolds are obtained by decellularization of native liver. Frequently used decellularization methods include immersion or peristaltic pump perfusion techniques. Immersion technique requires longer duration and risk of tissue decay in the tissue core. Limited laboratory facilities have a peristaltic pump, thus limiting the usage of perfusion decellularization techniques. Therefore, the aim of this study was to develop a technique of simple liver decellularization with multiple site syringe injection. Material and methods: Isolated rat livers were freeze-thawed and cut into cubical form with a size of 1.5 cm × 1.5 cm × 0.7 cm up to 1 cm. The liver cubes were incubated in EGTA solution for 30 minutes and followed by multiple site syringe injection with increasing SDS concentration (0.1 %, 0.25%, 0.5%, 0.75%, and 1%) until appeared transparent (± 6 hours). The decellularized liver cubes were processed histologically and stained with hematoxylin-eosin. Result and discussion: Decellularized liver cubes using multiple site syringe injection compared to undecellularized liver showed removal of cells and structurally intact extracellular matrix as observed from histological specimens. Conclusions: Multiple site syringe injection is a simple technique to decellularized liver cubes for preparation of native liver scaffold for tissue engineering.
AB - Scaffold is one of the important parts of tissue engineering. Liver scaffolds are obtained by decellularization of native liver. Frequently used decellularization methods include immersion or peristaltic pump perfusion techniques. Immersion technique requires longer duration and risk of tissue decay in the tissue core. Limited laboratory facilities have a peristaltic pump, thus limiting the usage of perfusion decellularization techniques. Therefore, the aim of this study was to develop a technique of simple liver decellularization with multiple site syringe injection. Material and methods: Isolated rat livers were freeze-thawed and cut into cubical form with a size of 1.5 cm × 1.5 cm × 0.7 cm up to 1 cm. The liver cubes were incubated in EGTA solution for 30 minutes and followed by multiple site syringe injection with increasing SDS concentration (0.1 %, 0.25%, 0.5%, 0.75%, and 1%) until appeared transparent (± 6 hours). The decellularized liver cubes were processed histologically and stained with hematoxylin-eosin. Result and discussion: Decellularized liver cubes using multiple site syringe injection compared to undecellularized liver showed removal of cells and structurally intact extracellular matrix as observed from histological specimens. Conclusions: Multiple site syringe injection is a simple technique to decellularized liver cubes for preparation of native liver scaffold for tissue engineering.
KW - biological liver scaffold
KW - liver decellularization
KW - liver tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85076760241&partnerID=8YFLogxK
U2 - 10.1063/1.5139367
DO - 10.1063/1.5139367
M3 - Conference contribution
AN - SCOPUS:85076760241
T3 - AIP Conference Proceedings
BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
A2 - Lischer, Kenny
A2 - Abuzairi, Tomy
A2 - Rahman, Siti Fauziyah
A2 - Gozan, Misri
PB - American Institute of Physics Inc.
Y2 - 22 July 2019 through 24 July 2019
ER -