Sistemic hypoxia causes lack of oxygen and energy in brain that trigger the release of acetylcholine,free radical and Glial fibrillary acidic protein (GFAP), a specific protein in astrocyte cells that act to strenghtenastrocite membrane. The aim of the research was to evaluate the damages of brain in systemic hypoxiathrough activity of acetylcholine esterase, neuron and astrocyte membran damages. The research conductedat the Chemistry and Molecular Biology Laboratory, FM Universitas Indonesia, in 2013. Twenty-five malespraque dawley rats were subjected to systemic hypoxia devided into 5 group of by placing them in thehypoxic chamber supplied 8-10% of O2 for 0, 1, 3, 5, and 7 days. Malondialdehyde (MDA), acetylcholineesterase specific activity and glial fibrillary acidic protein concentration were measured in plasma and braintissues. The result show that 7 day systemic hypoxia did not increase MDA concentration in plasma and braintissues, but increase significantly the spesific activity of acetylcholine esterase and GFAP concentration inbrain tissues. Systemic hypoxia as long as 7 day did not cause oxidative stress, but show increasing of AChEactivity and astrocyte adaptation through increasing of GFAP in brain tissues.
- hypoxia; astrocyte; malondialdehyde; acetylcholine esterase; glial fibrillary acidic protein.