TY - JOUR
T1 - Cytotoxicity and Antiobesity Activity of Freeze-Dried Malus domestica, Canarium sp. and Averrhoa bilimbi Fruit
AU - Utami, Sri
AU - Endrini, Susi
AU - Nafik, Said
AU - Arumwardana, Seila
AU - Rizal, Rizal
AU - Artie, Dwi Surya
AU - Yusepany, Dewani Tediana
AU - Kusuma, Hanna Sari Widya
AU - Widowati, Wahyu
N1 - Funding Information:
of Republic Indonesia, for the Research Grant of Hibah Bersaing/Penelitian Produk Terapan for financial support and gratefully acknowledge support from YARSI University. This research was also supported by the Biomolecular and Biomedical Research Center, Bandung, Indonesia. We are also thankful to Ika Adhani Sholihah, Alya Mardhotillah Azizah, Rr. Anisa Siwi Handayani, Riyani Lestari, Kamila Yashfa Gunawan, and Enden Dea Nataya for their valuable assistance.
Funding Information:
We are grateful to Directorate General for Higher Education, Ministry of National Education of Republic Indonesia, for the Research Grant of Hibah Bersaing/Penelitian Produk Terapan for financial support and gratefully acknowledge support from YARSI University. This research was also supported by the Biomolecular and Biomedical Research Center, Bandung, Indonesia. We are also thankful to Ika Adhani Sholihah, Alya Mardhotillah Azizah, Rr. Anisa Siwi Handayani, Riyani Lestari, Kamila Yashfa Gunawan, and Enden Dea Nataya for their valuable assistance.
Publisher Copyright:
© 2021, Universitas Gadjah Mada - Faculty of Pharmacy. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Obesity has a role in the development of diseases such as diabetes, cardiovascular disease, and hyperlipidemia which is characterized by the increase of adipose tissue mass due to an imbalance of energy intake and expenditure. Freeze-dried fruits are well known to possess antiobesity activity. In this study, we have evaluated the antiobesity activity of freeze-dried fruit (M. domestica, Canarium sp., and A. bilimbi) using CHOL, G6PDH, TG level, and Oil Red O assay. The viability of 3T3-L1 cell in the Canarium sp. freeze-dried in the concentration of 12.50 µg/ml has a higher value compared to M. domestica and A. bilimbi. The measurements of CHOL, G6PDH, TG level, and Oil Red O assay of the M. domestica freeze-dried in the concentration of 75 μg/ml has higher inhibitory activity compared to the Canarium sp. and A. bilimbi freeze-dried. In the CHOL assay, M. domestica freeze-dried has a higher value compared to A. bilimbi and Canarium sp. In the G6PDH assay, the freeze-dried of M. domestica has the value of 49.56%, Canarium sp. (45.22%), and A. bilimbi (47.13%), while in the Oil Red O assay, M. domestica has inhibition activity of 62.63%, A. bilimbi 50.01% and Canarium sp. 44.13%. The level of TG showed that M. domestica has higher activity with the value of 60.61%, A. bilimbi 57.54%, while Canarium sp. 55.03%. The freeze-dried of M. domestica in the concentration of 75 μg/ml has good inhibitory activity of lipid compared to A. bilimbi and Canarium sp.
AB - Obesity has a role in the development of diseases such as diabetes, cardiovascular disease, and hyperlipidemia which is characterized by the increase of adipose tissue mass due to an imbalance of energy intake and expenditure. Freeze-dried fruits are well known to possess antiobesity activity. In this study, we have evaluated the antiobesity activity of freeze-dried fruit (M. domestica, Canarium sp., and A. bilimbi) using CHOL, G6PDH, TG level, and Oil Red O assay. The viability of 3T3-L1 cell in the Canarium sp. freeze-dried in the concentration of 12.50 µg/ml has a higher value compared to M. domestica and A. bilimbi. The measurements of CHOL, G6PDH, TG level, and Oil Red O assay of the M. domestica freeze-dried in the concentration of 75 μg/ml has higher inhibitory activity compared to the Canarium sp. and A. bilimbi freeze-dried. In the CHOL assay, M. domestica freeze-dried has a higher value compared to A. bilimbi and Canarium sp. In the G6PDH assay, the freeze-dried of M. domestica has the value of 49.56%, Canarium sp. (45.22%), and A. bilimbi (47.13%), while in the Oil Red O assay, M. domestica has inhibition activity of 62.63%, A. bilimbi 50.01% and Canarium sp. 44.13%. The level of TG showed that M. domestica has higher activity with the value of 60.61%, A. bilimbi 57.54%, while Canarium sp. 55.03%. The freeze-dried of M. domestica in the concentration of 75 μg/ml has good inhibitory activity of lipid compared to A. bilimbi and Canarium sp.
KW - adipogenesis
KW - Antiobesity
KW - Averrhhoa bilimbi
KW - Canarium sp
KW - freeze-dried
KW - Malus domestica
UR - http://www.scopus.com/inward/record.url?scp=85154066949&partnerID=8YFLogxK
U2 - 10.22146/mot.52902
DO - 10.22146/mot.52902
M3 - Article
AN - SCOPUS:85154066949
SN - 1410-5918
VL - 26
SP - 21
EP - 27
JO - Majalah Obat Tradisional
JF - Majalah Obat Tradisional
IS - 1
ER -
