Cytotoxic secondary metabolites from fermentation broth of Brucea javanica endophytic fungus 1.2.11

Shirly Kumala, Robert Utji, Pratiwi Pudjilestari Sudarmono, L. B.S. Kardono

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2 Citations (Scopus)


Endophytic fungus 1.2.11 was isolated from the fruits of Brucea javanica (L.) Merr plant collected from Cianjur, West Java. The purpose of this study was to investigate the secondary metabolites from the fermentation broth of endophytic fungus 1.2.11 as anticancer potential. Liquid fermentation with Potato Dextrose Yeast extract (PDY) broth was carried out followed by incubation over 14 days in a shaking incubator. In vitro cytotoxic assay was performed using leukemia cell L1210. Viable cells were counted using Trypan blue method. The IC50 was obtained arithmetically using the Reed and Muench standard. Purification and isolation of cytotoxic secondary metabolites of the n-butanol extract were performed using gravity column chromatopgraphy and semi-preparative high performance liquid chromatography (HPLC) led to the F4-fraction as the active fraction. This fraction showed an IC50 of 4.29 μg mL-1. The LC-MS analysis of F4 showed a total ion chromatogram having five peaks, peak 1-5 as the secondary metabolites. The MS data of peaks 3 and 5 had molecular ions of m/z [M]+ 487 and 252, respectively. Peak 3 was proposed as a derivative of bruceocin and peak 5 was a canthin-6-one, respectively. Bruceocin and canthin-6-one were previously reported as the cytotoxic constituents from the B. javanica leaves. The current study demonstrated the possible endophytic fungi living symbiotically within the host plant B. javanica to produce cytotoxic secondary metabolites. One of the isolated fungi was fungus 1.2.11, identified as Fusarium chlamydosporum.

Original languageEnglish
Pages (from-to)625-631
Number of pages7
JournalResearch Journal of Microbiology
Issue number8
Publication statusPublished - 10 Sep 2007


  • Brucea javanica (L.) Merr
  • Cytotoxic
  • Endophytic fungus 1.2.11
  • Fusarium chlamydosporum
  • IC


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