Cytogenetic comparison of bioceramic, silicone, and methacrylate resin sealers on t lymphocytes (Micronuclei analysis)

Dewi Isroyati Sugiana, Kamizar Nazar, Ratna Meidyawati, Sunny Tulim

Research output: Contribution to journalArticle

Abstract

Objective: Biocompatibility refers to the manner in which materials respond to living cells and includes cytotoxicity, cytogenicity, genotoxicity, and carcinogenicity. To determine cytogenicity, we count the micronuclei that form after applying materials to living cells. Sealer is a chemical material that can be directly contacted in periapical tissue and is potentially cytogenetic. Bioceramic, silicon, and methacrylate resin sealers have ingredients that are potentially cytogenetic. We examined the interactions of these sealers with lymphocyte T-cells. Methods: We counted the number of micronuclei following treatment with bioceramic, silicone, and methacrylate resin sealers on lymphocyte T-cells at 1, 3, and 7 days. Results: The micronuclei scores associated with bioceramic and silicone sealers were lower than methacrylate resin (p<0.05) between days 1, 3, and 7. The micronuclei scores of bioceramic and silicone sealers on day 1 were higher than on days 3 and 7. There were no significant between-group differences for bioceramic and silicone sealers on days 3 and 7. The highest micronuclei score for methacrylate resin was on day 1. Conclusion: Bioceramic and silicone sealers were less cytogenetic than methacrylate resin sealer. However, all of the sealers produce micronuclei on days 1, 3, and 7.

Original languageEnglish
Pages (from-to)146-149
Number of pages4
JournalInternational Journal of Applied Pharmaceutics
Volume11
DOIs
Publication statusPublished - 1 Apr 2019

Keywords

  • Bioceramic
  • Cytogenetic
  • Methacrylate resin
  • Micronuclei
  • Silicone

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