TY - JOUR
T1 - Cyclea Barbata leaf extract
T2 - Lipoxygenase inhibitory activity and phytochemical screening
AU - Handayani, Nadya Febri
AU - Elya, Berna
AU - Puspitasari, Nuraini
N1 - Funding Information:
The authors would like to express gratitude and appreciation to Universitas Indonesia for financing this study through PITTA Research Grants Universitas Indonesia 2017.
Publisher Copyright:
© 2018 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2018/12
Y1 - 2018/12
N2 - Objective: The aim of this study was to test the anti-inflammatory activity of methanol, ethyl acetate, and n-hexane Cyclea barbata Miers. leaf extracts using a lipoxygenase inhibition method. Methods: Enzyme activity assay was performed using colorimetric method with lipoxygenase from soybean and linoleic acid as a substrate. Absorbance was measured at 234 nm. The total flavonoid content of the most active extract was determined using a colorimetric method with AlCl3 and phytochemical screening. Results: The ethyl acetate extract had the highest lipoxygenase inhibiting activity, with an IC50 value of 0.267 µg/mL. In addition, this extract had a total flavonoid content of 21.62 mgQE/g and contained flavonoids, glycosides, and terpenoids. Conclusion: This finding indicated that C. barbata provides a possible anti-inflammatory effect through the inhibition of lipoxygenase. However, further research is still needed to strengthen this result.
AB - Objective: The aim of this study was to test the anti-inflammatory activity of methanol, ethyl acetate, and n-hexane Cyclea barbata Miers. leaf extracts using a lipoxygenase inhibition method. Methods: Enzyme activity assay was performed using colorimetric method with lipoxygenase from soybean and linoleic acid as a substrate. Absorbance was measured at 234 nm. The total flavonoid content of the most active extract was determined using a colorimetric method with AlCl3 and phytochemical screening. Results: The ethyl acetate extract had the highest lipoxygenase inhibiting activity, with an IC50 value of 0.267 µg/mL. In addition, this extract had a total flavonoid content of 21.62 mgQE/g and contained flavonoids, glycosides, and terpenoids. Conclusion: This finding indicated that C. barbata provides a possible anti-inflammatory effect through the inhibition of lipoxygenase. However, further research is still needed to strengthen this result.
KW - Anti-inflammation
KW - Cyclea barbata miers. leaves
KW - Flavonoid content
KW - Lipoxygenase
KW - Phytochemical screening
UR - http://www.scopus.com/inward/record.url?scp=85071855765&partnerID=8YFLogxK
U2 - 10.22159/ijap.2018.v10s1.22
DO - 10.22159/ijap.2018.v10s1.22
M3 - Article
AN - SCOPUS:85071855765
SN - 0975-7058
VL - 10
SP - 106
EP - 109
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - Special Issue 1
ER -