Cutoff Value of Qualitative HBsAg for Confirmatory HBsAg Using the Chemiluminescence Microparticle Immunoassay Method

Merci Monica Pasaribu, Jessica Purwanti Wonohutomo, Suzanna Immanuel, July Kumalawati, Nuri Dyah Indrasari, Yusra Yusra

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Confirmatory hepatitis B surface antigen (HBsAg) is an assay used to distinguish weakly reactive from false-positive HBsAg results. OBJECTIVE: To determine the signal to cutoff (S/CO) value of chemiluminescence microparticle immunoassay (CMIA) HBsAg assay that should trigger follow-up confirmatory HBsAg testing. METHODS: All specimens with an initial S/CO value of 0.90-100.00 were subjected to repeat HBsAg testing after high-speed centrifugation. The specimens with an initial S/CO value in that range remained in the same range and were then followed up with confirmatory HBsAg testing. RESULT: In total, 132 specimens had an S/CO value between 0.90 and 100.00 after high-speed centrifugation, followed by confirmatory HBsAg retesting. The S/CO value of HBsAg specimens for which the results required verification with confirmatory HBsAg was 0.98 (100% sensitivity, 3.3% specificity) through 9.32 (47.1% sensitivity, 100% specificity). CONCLUSION: The HBsAg S/CO values (as determined by the chemiluminescent microparticle immunoassay [CMIA] method) that should trigger confirmatory HBsAg testing are 0.98-9.32.

Original languageEnglish
Pages (from-to)475-478
Number of pages4
JournalLaboratory Medicine
Volume53
Issue number5
DOIs
Publication statusPublished - 1 Sep 2022

Keywords

  • CMIA
  • false positive
  • HBsAg Qualitative II
  • HBsAg Qualitative II Confirmatory
  • hepatitis B
  • S/CO value

Fingerprint

Dive into the research topics of 'Cutoff Value of Qualitative HBsAg for Confirmatory HBsAg Using the Chemiluminescence Microparticle Immunoassay Method'. Together they form a unique fingerprint.

Cite this