TY - JOUR
T1 - Curcumin protects against failure to generate a transmembrane potential and protein aggregation of rat liver mitochondria induced by tert-butylhydroperoxides
AU - Suyatna, Franciscus D.
AU - Susilowati, S.
AU - Setiawati, A.
PY - 2007/7/1
Y1 - 2007/7/1
N2 - Curcumin, an active ingredient of curcuma plant has been thought to be beneficial in the treatment of liver diseases. In the previous studies, we have shown the protective effects of curcumin against lipid peroxidation and swelling of the rat liver mitochondrial preparation induced by tert-butylhydroperoxide (t-BuOOH). In the present study, the administration of t-BuOOH of 90 μM caused the mitochondria failed to generate a transmembrane potential (ψm). Of 3 doses of curcumin administered (0.5 μM, 2.5 μM dan 5.0 μM) the maximum protective effect against failure to generate a transmembrane potential caused by t-BuOOH was obtained by 2.5 μM of curcumin (79.13 ± 6.28%). Further, curcumin of 1000 μM could prevent protein aggregation formation caused by t-BuOOH in the electrophoretogram. This study shows the protective effects of curcumin against damaged of energy production system and protein of the mitochondria caused by t-BuOOH.
AB - Curcumin, an active ingredient of curcuma plant has been thought to be beneficial in the treatment of liver diseases. In the previous studies, we have shown the protective effects of curcumin against lipid peroxidation and swelling of the rat liver mitochondrial preparation induced by tert-butylhydroperoxide (t-BuOOH). In the present study, the administration of t-BuOOH of 90 μM caused the mitochondria failed to generate a transmembrane potential (ψm). Of 3 doses of curcumin administered (0.5 μM, 2.5 μM dan 5.0 μM) the maximum protective effect against failure to generate a transmembrane potential caused by t-BuOOH was obtained by 2.5 μM of curcumin (79.13 ± 6.28%). Further, curcumin of 1000 μM could prevent protein aggregation formation caused by t-BuOOH in the electrophoretogram. This study shows the protective effects of curcumin against damaged of energy production system and protein of the mitochondria caused by t-BuOOH.
KW - Curcumin
KW - Mitochondria
KW - Protein electrophoresis
KW - T-BuOOH
KW - Transmembrane potential
UR - http://www.scopus.com/inward/record.url?scp=85008951568&partnerID=8YFLogxK
U2 - 10.13181/mji.v16i3.269
DO - 10.13181/mji.v16i3.269
M3 - Article
AN - SCOPUS:85008951568
VL - 16
SP - 139
EP - 145
JO - Medical Journal of Indonesia
JF - Medical Journal of Indonesia
SN - 0853-1773
IS - 3
ER -