CURCUMIN AS AN ANTI-PROLIFERATIVE AGENT IN BREAST CANCER THROUGH RASSF1A, BAX, AND CASPASE-3 PROTEIN

N. A. Rahmah, H. Harliansyah, F. D. Suyatna, M. Kanoko, P. Rustamadji, J. Prihartono, A. Bustami, S. J. Haryono, B. S. Hernowo

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specific mechanism of curcumin cytotoxicity remains controversial because of many anti-and pro-apoptotic signaling pathways in various cell types. This study aims to examine the relationship among curcumin on RASSF1A, Bax protein levels, and caspase-3 activity in supporting the apoptotic mechanism in CSA03 breast cancer cells. Material and Methods. Curcumin administration to cancer cells is based on differences in dosage with 24-hour incubation. Cytotoxicity after curcumin administration was determined using MTS. RASSF1A and Bax protein levels were tested through ELISA. Caspase-3 activity was used to determine apoptosis and was tested using flow cytometry. Results. The results indicated that curcumin had a cytotoxicity effect of 40.85 µg/mL. At concentrations of 40 µg/mL and 50 µg/mL, curcumin increases levels of protein RASSF1A (∆ = 26.53% and 47.35%, respectively), Bax (∆ = 48.79% and 386.15%), and caspase-3 (∆ = 1,678.51% and 1,871.889%) significantly. Conclusions. Curcumin exhibits anti-proliferative activity and apoptotic (Caspase-3) effects through activation of RASSF1A and Bax.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalSiberian Journal of Oncology
Volume21
Issue number6
DOIs
Publication statusPublished - 2022

Keywords

  • Bax
  • caspase-3
  • CSA03
  • curcumin
  • RASSF1A

Fingerprint

Dive into the research topics of 'CURCUMIN AS AN ANTI-PROLIFERATIVE AGENT IN BREAST CANCER THROUGH RASSF1A, BAX, AND CASPASE-3 PROTEIN'. Together they form a unique fingerprint.

Cite this