Crude fucoidan activity extracted from Sargassum sp. as mycotoxin (T-2) binder

Marta Yusfita Sari, Endang Saepudin, Fitri Fegatella

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fucoidan is a sulfated polysaccharide which is mainly found in brown seaweed like Sargassum sp. In this study, fucoidan was extracted with two distinct methods, one was using 0.1 N HCl (Fucoidan A) and the other 0.1 N H3PO4 (Fucoidan B). Yields of HCl and H3PO4 extractions were 7.50 and 7.02%, respectively. Characterization of crude fucoidan was carried out through Fourier-transform infrared spectroscopy (FTIR), followed by total carbohydrate and total sulfate level measurement. Crude fucoidan was then used for the determination of its binding activity against Trichothecenes T-2 toxin. Quantitative analysis of 50 µg/mL T-2 toxin binding capacity shows the efficiency of 59.52% at pH 3.0 and 58.37% at pH 6.8 for crude fucoidan A. As for crude fucoidan B, efficiency of T-2 toxin binding capacity has value of 58.12% at pH 3.0 and 57.33% at pH 6.8. Meanwhile, Commercial crude fucoidan extract from Fucus vesiculosus sp. was used as control for T-2 toxin binding capacity analysis with efficiency of 57.31% at pH 3.0 and 56.64% at pH 6.8. Therefore, fucoidan from Sargassum sp. can be utilized better as mycotoxin especially T-2 toxin binder than from Fucus vesiculosus sp. through efficiency result of fucoidan binding capacity.

Original languageEnglish
Title of host publicationSymposium of Materials Science and Chemistry II
EditorsTutik Dwi Wahyuningsih, Roto Roto, Dwi Siswanta, Rohana Adnan, Laurent Commeiras, Kuwat Triyana, Kuwat Triyana, Indriana Kartini, Julius Motuzas
PublisherTrans Tech Publications Ltd
Pages193-198
Number of pages6
ISBN (Print)9783035716139
Publication statusPublished - 1 Jan 2020
Event5th International Conference on Science and Technology, ICST 2019 - Yogyakarta, Indonesia
Duration: 30 Jul 201931 Jul 2019

Publication series

NameKey Engineering Materials
Volume840 KEM
ISSN (Print)1013-9826
ISSN (Electronic)1662-9795

Conference

Conference5th International Conference on Science and Technology, ICST 2019
CountryIndonesia
CityYogyakarta
Period30/07/1931/07/19

Keywords

  • Fucoidan
  • Mycotoxin
  • Sargassum sp.
  • T-2 toxin
  • Trichothecenes

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  • Cite this

    Sari, M. Y., Saepudin, E., & Fegatella, F. (2020). Crude fucoidan activity extracted from Sargassum sp. as mycotoxin (T-2) binder. In T. D. Wahyuningsih, R. Roto, D. Siswanta, R. Adnan, L. Commeiras, K. Triyana, K. Triyana, I. Kartini, & J. Motuzas (Eds.), Symposium of Materials Science and Chemistry II (pp. 193-198). (Key Engineering Materials; Vol. 840 KEM). Trans Tech Publications Ltd.