TY - JOUR
T1 - Cosmetic serum containing grape (Vitis vinifera L.) seed extract phytosome
T2 - Formulation and in vitro penetration study
AU - Surini, Silvia
AU - Mubarak, Helmy
AU - Ramadon, Delly
N1 - Publisher Copyright:
© 2018 EManuscript Technologies. All rights reserved.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Objective: Grape seed extract (GSE) contains abundant phenolic compounds with high antioxidant activity. Phenolic compounds in GSE have an inadequate penetration due to its hydrophilicity. The objective of this research was to formulate GSE phytosome serum to overcome its penetration problem. Methods: Phytosomes were prepared in three formulas based on the mass ratio between the extract and the phosphatidylcholine, 1:0.5, 1:1, and 1:2, using the thin layer hydration method. Phytosomes were then characterized their morphology, particle size distribution, zeta potential and their entrapment efficiency. The selected phytosome was formulated into a gel-based serum.Then, physicochemical evaluations of the serum, including organoleptic test (color, odor, and syneresis), homogeneity, pH, viscosity and rheology properties, were conducted. An in vitro penetration study using the Franz diffusion cells was performed on phytosome and non-phytosome serum (as a control). Results: The results showed that F2 was the optimal phytosome among other formula with a spherical shape, Dmean volume was 398.23 nm, zeta potential at -25.2 mV and entrapment efficiency of 75.01±0.25%. The cumulative amount of total phenolic penetrated from the phytosome serum and control were 27.25±0.67% and 11.97±0.49%, respectively. The phytosome serum had a flux value of 243.11±7.94 μg/cm2.hour, while the control serum was 68.56±5.54 μg/cm2. hour. Conclusion: This research demonstrated that phytosome could increase the penetration of the drug in serum dosage form.
AB - Objective: Grape seed extract (GSE) contains abundant phenolic compounds with high antioxidant activity. Phenolic compounds in GSE have an inadequate penetration due to its hydrophilicity. The objective of this research was to formulate GSE phytosome serum to overcome its penetration problem. Methods: Phytosomes were prepared in three formulas based on the mass ratio between the extract and the phosphatidylcholine, 1:0.5, 1:1, and 1:2, using the thin layer hydration method. Phytosomes were then characterized their morphology, particle size distribution, zeta potential and their entrapment efficiency. The selected phytosome was formulated into a gel-based serum.Then, physicochemical evaluations of the serum, including organoleptic test (color, odor, and syneresis), homogeneity, pH, viscosity and rheology properties, were conducted. An in vitro penetration study using the Franz diffusion cells was performed on phytosome and non-phytosome serum (as a control). Results: The results showed that F2 was the optimal phytosome among other formula with a spherical shape, Dmean volume was 398.23 nm, zeta potential at -25.2 mV and entrapment efficiency of 75.01±0.25%. The cumulative amount of total phenolic penetrated from the phytosome serum and control were 27.25±0.67% and 11.97±0.49%, respectively. The phytosome serum had a flux value of 243.11±7.94 μg/cm2.hour, while the control serum was 68.56±5.54 μg/cm2. hour. Conclusion: This research demonstrated that phytosome could increase the penetration of the drug in serum dosage form.
KW - Franz diffusion Cell
KW - Grape seed extract
KW - Penetration
KW - Phytosome
KW - Serum
KW - Total phenolic
UR - http://www.scopus.com/inward/record.url?scp=85050287029&partnerID=8YFLogxK
U2 - 10.5530/jyp.2018.2s.10
DO - 10.5530/jyp.2018.2s.10
M3 - Article
AN - SCOPUS:85050287029
SN - 0975-1483
VL - 10
SP - s51-s55
JO - Journal of Young Pharmacists
JF - Journal of Young Pharmacists
IS - 2
ER -