Correlation of noise intensity to Heat Shock Response with Hsp 70, p53, Cytochrome C, Caspase 3 expressions and ultrastructure region of Rattus norvegicus’s cochlea

R. Yusa Herwanto, Jenny Bashiruddin, Syafruddin Ilyas, M. Nadjib Dahlan Lubis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Method: Hsp 70, p53, Cytoch.ome C and Caspase 3 expression are the specific protein markers due to the continuous environment noise exposure. Trearment consist of 5 groups are; P0 = control group/noise <25 dB; P1 = noise by 30-50 dB; P2 = noise by 60-70 dB; P3 = noisy at 80-90 dB and P4 = noisy at 100-110 dB. Each groups has been done for 8 days and 8 hours/day. Invivo experimental method with white rat Rattus norvegicus for the purpose to measure the stress protein marker in cochlea tissue with Hsp 70, p53, Cytochrome C and Caspase 3 to evaluate the apoptosis of the involved cochlea. Cochlea structure with Scanning Electron Microscope (SEM) has been done after the noise various intensity for 8 hours per day as long as 8 days and was terminated.

Introduction: Noise is one of the stress factors that mostly found in our environment. The regular and continuous noise exposure will cause permanent damage to cochlea as hearing organ.

Results: This study shown that Hsp 70 expression tend to increased due to P0-P4, but other markers tight increased such as expression of p53, Cytochrome C and Caspase 3 with the score 4-6 (moderate intensity). All apoptosis biomarker groups have shown significant difference compare to experiment group (p<0.01). The results of SEM on the organ of corti at the group P1 to P4 showed the gradual destruction (P4>50%).

Original languageEnglish
Pages (from-to)80-84
Number of pages5
JournalInternational Journal of PharmTech Research
Volume7
Issue number1
Publication statusPublished - 1 Jan 2015

Keywords

  • Caspase 3
  • Cytochrome C
  • Hsp 70
  • Noise
  • Organ of corti
  • P53

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