Correlation of mir-150, hsa-let-7e, and mir-146a and gene expression of il-6, il-8, ip-10, and mip-1β during dengue virus infection

Sri Masyeni, Kuntaman Kuntaman, Aryati Aryati, Muchlis Achsan Udji Sofro, Usman Hadi, Gondo Mastutik, Windu Purnomo, Agus Santosa, Benediktus Yohan, Erni Juwita Nelwan, R. Tedjo Sasmono

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR- 150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let- 7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.

Original languageEnglish
Article numbere31
JournalNarra J
Volume1
Issue number1
DOIs
Publication statusPublished - Apr 2021

Keywords

  • Dengue
  • Hsa-let-7e
  • MicroRNA
  • MiR-146a
  • MiR-150

Fingerprint

Dive into the research topics of 'Correlation of mir-150, hsa-let-7e, and mir-146a and gene expression of il-6, il-8, ip-10, and mip-1β during dengue virus infection'. Together they form a unique fingerprint.

Cite this