Conversion of paper waste to bioethanol using selected enzyme combination (cellulase and cellobiase) through simultaneous saccharification and fermentation

Muhammad Arif Darmawan, Yulis Aswar Hermawan, M. Samsuri, Misri Gozan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

One of the relatively inexpensive alternative energy sources in terms of production and relatively environment friendly was the development of bioethanol from urban wastes that contain many lignocellulose such as paper waste. This study characterizes the composition of cellulose, hemicellulose and lignin in blank HVS paper, inked HVS and newspaper. The results showed the predominate cellulose (49-60 %) while the lignin concentration was very low due to the removal of colors (lignin) in the manufacturing process. The experiments continued with synthesis of bioethanol through Simultaneous Saccharification and Fermentation (SSF) process. A combination of enzymes (Cellulase + Cellobiase) in 1:1 weight ratio and Saccharomyces cerevisiae was used for hydrolysis and fermentation in the SSF process. Paper waste from newspapers, inked HVS, and empty HVS papers were used as raw materials. The initial pH of SSF in the column was at pH 5. Variation of enzyme concentration was carried at 0.2, 0.3 and 0.5g. The SSF process was carried out at incubation time of 6, 12, 24, 36, 48, 72, and 96 hours. The ink affected the concentration of bioethanol produced. In this study, the best bioethanol content was produced by inked HVS and Newspapers at 0.5 grams of enzyme with a concentration of 1531 and 1111ppm, respectively.

Original languageEnglish
Title of host publication11th Regional Conference on Chemical Engineering, RCChE 2018
EditorsTeguh Ariyanto, Imam Prasetyo, Rochmadi, Nur Rofiqoh Eviana Putri
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418158
DOIs
Publication statusPublished - 21 Mar 2019
Event11th Regional Conference on Chemical Engineering, RCChE 2018 - Yogyakarta, Indonesia
Duration: 7 Nov 20188 Nov 2018

Publication series

NameAIP Conference Proceedings
Volume2085
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference11th Regional Conference on Chemical Engineering, RCChE 2018
CountryIndonesia
CityYogyakarta
Period7/11/188/11/18

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    Darmawan, M. A., Hermawan, Y. A., Samsuri, M., & Gozan, M. (2019). Conversion of paper waste to bioethanol using selected enzyme combination (cellulase and cellobiase) through simultaneous saccharification and fermentation. In T. Ariyanto, I. Prasetyo, Rochmadi, & N. R. E. Putri (Eds.), 11th Regional Conference on Chemical Engineering, RCChE 2018 [020018] (AIP Conference Proceedings; Vol. 2085). American Institute of Physics Inc.. https://doi.org/10.1063/1.5094996