TY - JOUR
T1 - Construction of Recombinant Plasmid pcDNA3.1/BMP-2 and its Involvement in Differentiation of Human Dental Pulp-Derived Cells into an Odontoblastic Lineage
AU - Joenoes, Hedijanti
AU - Yuniastuti, Mindia
AU - Bachtiar, Endang W.
AU - Bachtiar, Boy M.
PY - 2009
Y1 - 2009
N2 - Experimental studies have shown that dental pulp tissue has potential to regenerate dentine in response to adverse stimuli, such as caries and associated operative procedures. However, the potential of dental pulp regeneration seems to be limited by regenerative capacity of the cell involved. In this study, we report the effect of transfection of a recombinant plasmid containing human BMP-2 gene in proliferation and differentiation of dental pulp tissue in vitro. The regenerative capacity was analyzed by ALP production and calcium content. Results showed that the transfected dental pulp cell was able to differentiate into the odontoblast phenotype, indicating the presence of odontoblast progentitor cells in dental pulp tissue.
AB - Experimental studies have shown that dental pulp tissue has potential to regenerate dentine in response to adverse stimuli, such as caries and associated operative procedures. However, the potential of dental pulp regeneration seems to be limited by regenerative capacity of the cell involved. In this study, we report the effect of transfection of a recombinant plasmid containing human BMP-2 gene in proliferation and differentiation of dental pulp tissue in vitro. The regenerative capacity was analyzed by ALP production and calcium content. Results showed that the transfected dental pulp cell was able to differentiate into the odontoblast phenotype, indicating the presence of odontoblast progentitor cells in dental pulp tissue.
UR - http://journal.ui.ac.id/index.php/health/article/view/327
U2 - 10.7454/msk.v13i1.327
DO - 10.7454/msk.v13i1.327
M3 - Article
SN - 2356-3664
VL - 13
JO - Makara Journal of Health Research
JF - Makara Journal of Health Research
IS - 1
ER -