Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure

Dwi Endah; Intan Cahaya Dani

doi:10.1063/5.0133848

Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure

Dwi Endah, Budiawan, Intan Cahaya Dani

Department of Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

International Agency for Research on Cancer (IARC) has been classified acrylamide as "probably carcinogenic to humans"(group 2A). Acrylamide exposure in high concentration induced reactive oxygen species and generated 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) leading to oxidative DNA damage. This study aimed to detect DNA Adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker oxidative DNA damage caused by reactive oxygen species from acrylamide exposure. This in vitro study was conducted by incubating 2-deoxyguanosine, one of the bases DNA, H2O2, and acrylamide as xenobiotic. The reaction was incubated at 37 °C for 24 hours and varied at pH 7,4 and 8,4. 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) was analyzed by using reverse-phase HPLC with UV-Vis detector and an equipped mixture of phosphate buffer and methanol as mobile phase. The results showed that acrylamide could interact with DNA base, 2-deoxyguanosine, and induced 8-OHdG formation. The 8-OHdG concentration was higher from the exposure of 2-deoxyguanosine to acrylamide and H2O2 compared to the reaction without the addition of acrylamide. This result indicated that acrylamide exposure probably contributed to ROS formation and lead to the higher 8-OHdG level. The higher pH also induced the higher 8-OHdG formation.

Original language	English
Title of host publication	2nd International Conference on Chemistry and Science Education
Subtitle of host publication	Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution
Editors	Guspatni, Zonalia Fitriza, Budhi Oktavia
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735444522
DOIs	https://doi.org/10.1063/5.0133848
Publication status	Published - 24 Apr 2023
Event	2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution, ICChSE 2021 - Virtual, Online Duration: 4 Aug 2021 → 5 Aug 2021

Publication series

Name	AIP Conference Proceedings
Volume	2673
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution, ICChSE 2021
City	Virtual, Online
Period	4/08/21 → 5/08/21

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1063/5.0133848

Cite this

Endah, D., Budiawan, & Dani, I. C. (2023). Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure. In Guspatni, Z. Fitriza, & B. Oktavia (Eds.), 2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution Article 020002 (AIP Conference Proceedings; Vol. 2673). American Institute of Physics Inc.. https://doi.org/10.1063/5.0133848

Endah, Dwi ; Budiawan ; Dani, Intan Cahaya. / Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure. 2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution. editor / Guspatni ; Zonalia Fitriza ; Budhi Oktavia. American Institute of Physics Inc., 2023. (AIP Conference Proceedings).

@inproceedings{ea6970c1eb90418ca2b0a1891b7a713b,

title = "Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure",

abstract = "International Agency for Research on Cancer (IARC) has been classified acrylamide as {"}probably carcinogenic to humans{"}(group 2A). Acrylamide exposure in high concentration induced reactive oxygen species and generated 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) leading to oxidative DNA damage. This study aimed to detect DNA Adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker oxidative DNA damage caused by reactive oxygen species from acrylamide exposure. This in vitro study was conducted by incubating 2-deoxyguanosine, one of the bases DNA, H2O2, and acrylamide as xenobiotic. The reaction was incubated at 37 °C for 24 hours and varied at pH 7,4 and 8,4. 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) was analyzed by using reverse-phase HPLC with UV-Vis detector and an equipped mixture of phosphate buffer and methanol as mobile phase. The results showed that acrylamide could interact with DNA base, 2-deoxyguanosine, and induced 8-OHdG formation. The 8-OHdG concentration was higher from the exposure of 2-deoxyguanosine to acrylamide and H2O2 compared to the reaction without the addition of acrylamide. This result indicated that acrylamide exposure probably contributed to ROS formation and lead to the higher 8-OHdG level. The higher pH also induced the higher 8-OHdG formation.",

author = "Dwi Endah and Budiawan and Dani, {Intan Cahaya}",

note = "Publisher Copyright: {\textcopyright} 2023 Author(s).; 2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution, ICChSE 2021 ; Conference date: 04-08-2021 Through 05-08-2021",

year = "2023",

month = apr,

day = "24",

doi = "10.1063/5.0133848",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Guspatni and Zonalia Fitriza and Budhi Oktavia",

booktitle = "2nd International Conference on Chemistry and Science Education",

address = "United States",

}

Endah, D, Budiawan & Dani, IC 2023, Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure. in Guspatni, Z Fitriza & B Oktavia (eds), 2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution., 020002, AIP Conference Proceedings, vol. 2673, American Institute of Physics Inc., 2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution, ICChSE 2021, Virtual, Online, 4/08/21. https://doi.org/10.1063/5.0133848

Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure. / Endah, Dwi; Budiawan; Dani, Intan Cahaya.
2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution. ed. / Guspatni; Zonalia Fitriza; Budhi Oktavia. American Institute of Physics Inc., 2023. 020002 (AIP Conference Proceedings; Vol. 2673).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure

AU - Endah, Dwi

AU - Budiawan, null

AU - Dani, Intan Cahaya

PY - 2023/4/24

Y1 - 2023/4/24

N2 - International Agency for Research on Cancer (IARC) has been classified acrylamide as "probably carcinogenic to humans"(group 2A). Acrylamide exposure in high concentration induced reactive oxygen species and generated 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) leading to oxidative DNA damage. This study aimed to detect DNA Adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker oxidative DNA damage caused by reactive oxygen species from acrylamide exposure. This in vitro study was conducted by incubating 2-deoxyguanosine, one of the bases DNA, H2O2, and acrylamide as xenobiotic. The reaction was incubated at 37 °C for 24 hours and varied at pH 7,4 and 8,4. 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) was analyzed by using reverse-phase HPLC with UV-Vis detector and an equipped mixture of phosphate buffer and methanol as mobile phase. The results showed that acrylamide could interact with DNA base, 2-deoxyguanosine, and induced 8-OHdG formation. The 8-OHdG concentration was higher from the exposure of 2-deoxyguanosine to acrylamide and H2O2 compared to the reaction without the addition of acrylamide. This result indicated that acrylamide exposure probably contributed to ROS formation and lead to the higher 8-OHdG level. The higher pH also induced the higher 8-OHdG formation.

AB - International Agency for Research on Cancer (IARC) has been classified acrylamide as "probably carcinogenic to humans"(group 2A). Acrylamide exposure in high concentration induced reactive oxygen species and generated 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) leading to oxidative DNA damage. This study aimed to detect DNA Adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker oxidative DNA damage caused by reactive oxygen species from acrylamide exposure. This in vitro study was conducted by incubating 2-deoxyguanosine, one of the bases DNA, H2O2, and acrylamide as xenobiotic. The reaction was incubated at 37 °C for 24 hours and varied at pH 7,4 and 8,4. 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) was analyzed by using reverse-phase HPLC with UV-Vis detector and an equipped mixture of phosphate buffer and methanol as mobile phase. The results showed that acrylamide could interact with DNA base, 2-deoxyguanosine, and induced 8-OHdG formation. The 8-OHdG concentration was higher from the exposure of 2-deoxyguanosine to acrylamide and H2O2 compared to the reaction without the addition of acrylamide. This result indicated that acrylamide exposure probably contributed to ROS formation and lead to the higher 8-OHdG level. The higher pH also induced the higher 8-OHdG formation.

UR - http://www.scopus.com/inward/record.url?scp=85159887344&partnerID=8YFLogxK

U2 - 10.1063/5.0133848

DO - 10.1063/5.0133848

M3 - Conference contribution

AN - SCOPUS:85159887344

T3 - AIP Conference Proceedings

BT - 2nd International Conference on Chemistry and Science Education

A2 - Guspatni, null

A2 - Fitriza, Zonalia

A2 - Oktavia, Budhi

PB - American Institute of Physics Inc.

T2 - 2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution, ICChSE 2021

Y2 - 4 August 2021 through 5 August 2021

ER -

Endah D, Budiawan, Dani IC. Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure. In Guspatni, Fitriza Z, Oktavia B, editors, 2nd International Conference on Chemistry and Science Education: Improving the Research Quality in Chemistry and Natural Science Education for the Upcoming Challenges to Face the 5th Industrial Revolution. American Institute of Physics Inc. 2023. 020002. (AIP Conference Proceedings). doi: 10.1063/5.0133848

Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure

Abstract

Publication series

Conference

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this