TY - JOUR
T1 - Concentration dependent effects of carboxymethyl chitosan on dentin remineralization with amorphous calcium phosphate
AU - Setiati, Hasti Dwi
AU - Suprastiwi, Endang
AU - Artiningsih, Dewa Ayu Nyoman Putri
AU - Utami, Luh Putu Trisna Budi
N1 - Funding Information:
This research was supported by the Directorate of Research and Community Engagement of Universitas Indonesia, funded by PITTA grant funds. We thank our colleagues from the Department of Dental Material and the Environmental Sciences Laboratory in Universitas Indonesia.
Publisher Copyright:
© 2020 The Authors.
PY - 2020/7
Y1 - 2020/7
N2 - Objective: Carboxymethyl chitosan (CMC) is a non-collagenous protein analog which has a similar role as dentin matrix protein 1. CMC stabilizes amorphous calcium phosphate (ACP); hence forming nanocomplexes of CMC-ACP. The purpose of this study was to evaluate the effect of CMC concentration in CMC-ACP on dentin remineralization. Methods: Cavities were formed on the occlusal surfaces of freshly extracted premolar teeth. All samples were demineralized and immersed in phosphate-buffered saline and stored in a shaking incubator at 37°C. The teeth were randomly divided into five groups. Group 1 was control group (no treatment), whereas Groups 2, 3, 4, and 5 were treated with CMC-ACP containing 1%, 2.5%, 5%, and 10% CMC. The remineralized layer on the dentin surface was evaluated using scanning electron microscopy and energy-dispersive X-ray analysis. Results: The highest dentin remineralization capacity was achieved in Group 5 (10% CMC), whereas diminishing effects were observed in Group 4 (5% CMC), Group 3 (2.5% CMC), and Group 2 (1% CMC). Although no significant differences in calcium levels were observed between 2.5%, 5%, and 10% CMC groups, phosphate levels differed significantly in all treatment groups. Conclusion: Optimal dentin remineralization was achieved by the application of CMC–ACP containing 2.5% CMC.
AB - Objective: Carboxymethyl chitosan (CMC) is a non-collagenous protein analog which has a similar role as dentin matrix protein 1. CMC stabilizes amorphous calcium phosphate (ACP); hence forming nanocomplexes of CMC-ACP. The purpose of this study was to evaluate the effect of CMC concentration in CMC-ACP on dentin remineralization. Methods: Cavities were formed on the occlusal surfaces of freshly extracted premolar teeth. All samples were demineralized and immersed in phosphate-buffered saline and stored in a shaking incubator at 37°C. The teeth were randomly divided into five groups. Group 1 was control group (no treatment), whereas Groups 2, 3, 4, and 5 were treated with CMC-ACP containing 1%, 2.5%, 5%, and 10% CMC. The remineralized layer on the dentin surface was evaluated using scanning electron microscopy and energy-dispersive X-ray analysis. Results: The highest dentin remineralization capacity was achieved in Group 5 (10% CMC), whereas diminishing effects were observed in Group 4 (5% CMC), Group 3 (2.5% CMC), and Group 2 (1% CMC). Although no significant differences in calcium levels were observed between 2.5%, 5%, and 10% CMC groups, phosphate levels differed significantly in all treatment groups. Conclusion: Optimal dentin remineralization was achieved by the application of CMC–ACP containing 2.5% CMC.
KW - Amorphous calcium phosphate
KW - Carboxymethyl chitosan
KW - Demineralized dentin
KW - Non-collagenous protein analog
UR - http://www.scopus.com/inward/record.url?scp=85089439970&partnerID=8YFLogxK
U2 - 10.22159/ijap.2020.v12s2.OP-42
DO - 10.22159/ijap.2020.v12s2.OP-42
M3 - Article
AN - SCOPUS:85089439970
SN - 0975-7058
VL - 12
SP - 31
EP - 33
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - Special Issue 2
ER -