TY - GEN
T1 - Comparison study of human mononuclear cells presence in rat blood using flow cytometry and polymerase chain reaction
AU - Fasha, Iqbal
AU - Liem, Isabella Kurnia
AU - Antarianto, Radiana D.
AU - Sianipar, Imelda Rosalyn
AU - Yunaini, Luluk
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/9
Y1 - 2019/4/9
N2 - Perspectives on cell therapy using multipotent stem cells or progenitor stem cells are progressing rapidly. Therefore, biomedical research on cells detection method should in line with it. Flow cytometry technique is usually used quantitatively to see the presence of stem cells in different sources, such as blood, bone marrow, tissues, etc. Nevertheless, there is another modality that can be used as an alternative, i.e. polymerase chain reaction (PCR). This study aimed to know whether PCR could be used as an alternative method for replacing flow cytometry technique in detecting human mononuclear cells (hMNCs) derived from umbilical cord blood in the rat blood. Human mononuclear cells from umbilical cord blood were injected into rat tail's vein. Blood sampling was collected from the heart 30 minutes, 1 hour, 3 hours, 6 hours, 1 day and 7 days after injection. The blood was then analyzed with flow cytometry to determine the presence of hMNCs (CD34+ hMNCs) and PCR to determine the presence of human DNA (human chromosome 17 alpha satellite DNA; HSA17). Polymerase chain reaction could be detecting the presence of human DNA in all samples that are compared to the flow cytometry that also detect the presence of hMNCs in all samples. The results showed that flow cytometry and PCR technique have a similar trend pattern based on time dependent. Polymerase chain reaction techniques can be used as an alternative method in detecting the presence of hMNCs in the rat's blood equally to the flow cytometry.
AB - Perspectives on cell therapy using multipotent stem cells or progenitor stem cells are progressing rapidly. Therefore, biomedical research on cells detection method should in line with it. Flow cytometry technique is usually used quantitatively to see the presence of stem cells in different sources, such as blood, bone marrow, tissues, etc. Nevertheless, there is another modality that can be used as an alternative, i.e. polymerase chain reaction (PCR). This study aimed to know whether PCR could be used as an alternative method for replacing flow cytometry technique in detecting human mononuclear cells (hMNCs) derived from umbilical cord blood in the rat blood. Human mononuclear cells from umbilical cord blood were injected into rat tail's vein. Blood sampling was collected from the heart 30 minutes, 1 hour, 3 hours, 6 hours, 1 day and 7 days after injection. The blood was then analyzed with flow cytometry to determine the presence of hMNCs (CD34+ hMNCs) and PCR to determine the presence of human DNA (human chromosome 17 alpha satellite DNA; HSA17). Polymerase chain reaction could be detecting the presence of human DNA in all samples that are compared to the flow cytometry that also detect the presence of hMNCs in all samples. The results showed that flow cytometry and PCR technique have a similar trend pattern based on time dependent. Polymerase chain reaction techniques can be used as an alternative method in detecting the presence of hMNCs in the rat's blood equally to the flow cytometry.
KW - CD34+ cells
KW - flow cytometry
KW - HSA17
KW - human mononuclear cells
KW - umbilical cord blood
UR - http://www.scopus.com/inward/record.url?scp=85064844895&partnerID=8YFLogxK
U2 - 10.1063/1.5096714
DO - 10.1063/1.5096714
M3 - Conference contribution
AN - SCOPUS:85064844895
T3 - AIP Conference Proceedings
BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Wulan, Praswasti P.D.K.
A2 - Gozan, Misri
A2 - Astutiningsih, Sotya
A2 - Ramahdita, Ghiska
A2 - Dhelika, Radon
A2 - Kreshanti, Prasetyanugraheni
PB - American Institute of Physics Inc.
T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Y2 - 6 August 2018 through 8 August 2018
ER -