Comparison study of human mononuclear cells presence in rat blood using flow cytometry and polymerase chain reaction

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Perspectives on cell therapy using multipotent stem cells or progenitor stem cells are progressing rapidly. Therefore, biomedical research on cells detection method should in line with it. Flow cytometry technique is usually used quantitatively to see the presence of stem cells in different sources, such as blood, bone marrow, tissues, etc. Nevertheless, there is another modality that can be used as an alternative, i.e. polymerase chain reaction (PCR). This study aimed to know whether PCR could be used as an alternative method for replacing flow cytometry technique in detecting human mononuclear cells (hMNCs) derived from umbilical cord blood in the rat blood. Human mononuclear cells from umbilical cord blood were injected into rat tail's vein. Blood sampling was collected from the heart 30 minutes, 1 hour, 3 hours, 6 hours, 1 day and 7 days after injection. The blood was then analyzed with flow cytometry to determine the presence of hMNCs (CD34+ hMNCs) and PCR to determine the presence of human DNA (human chromosome 17 alpha satellite DNA; HSA17). Polymerase chain reaction could be detecting the presence of human DNA in all samples that are compared to the flow cytometry that also detect the presence of hMNCs in all samples. The results showed that flow cytometry and PCR technique have a similar trend pattern based on time dependent. Polymerase chain reaction techniques can be used as an alternative method in detecting the presence of hMNCs in the rat's blood equally to the flow cytometry.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
CountryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • CD34+ cells
  • flow cytometry
  • HSA17
  • human mononuclear cells
  • umbilical cord blood

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  • Cite this

    Fasha, I., Liem, I. K., Antarianto, R. D., Sianipar, I. R., & Yunaini, L. (2019). Comparison study of human mononuclear cells presence in rat blood using flow cytometry and polymerase chain reaction. In P. P. D. K. Wulan, M. Gozan, S. Astutiningsih, G. Ramahdita, R. Dhelika, & P. Kreshanti (Eds.), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018 [030010] (AIP Conference Proceedings; Vol. 2092). American Institute of Physics Inc.. https://doi.org/10.1063/1.5096714