Comparison of two dot immunoassays for diagnosis of dengue infection

Agus Sjahrurachman, Betty Emawati, Fera Lbrahim, Mardiastuti, Tjahjani Mirawati Sudiro, Pratiwi Pudjilestari Sudarmono

Research output: Contribution to journalArticlepeer-review

Abstract

Definite diagnosis of dengue infection plays an important role of case management and disease control. Unfortunately, majority of the infected cases can not be diagnosed solely on the basis of clinical signs and symptoms. Laboratory investigations are required. Since dengue distribution mostly present in developing countries, appropriate laboratory diagnosis would be a rapid and simple one. Here, we report a preliminary assessment of a dot immunoassay employing biotinylated-dengue antigens for detection of IgM-antidengue antibodies which is simple in term of methodology and rapid in term of the test result. This study was done by comparing out method with standard H.I test and commercially available dot immunoassay. The results indicate that the dot immunoassay employing biotinylated-dengue (BD1A) is sensitive and specific for diagnosis of dengue infection.

Original languageEnglish
Pages (from-to)248-252
Number of pages5
JournalMedical Journal of Indonesia
Volume9
Issue number4
DOIs
Publication statusPublished - 1 Oct 2000

Keywords

  • Dengue infection
  • Diagnosis
  • Qualitative immunoassay

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