Comparison of Real-Time BIOCOV-19 RT-PCR Kit with N1N2 CDC RT-PCR for Detecting SARS-COV-2

Yulia Rosa Saharman, Chairinda Dachwan, Andi Yasmon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that cause coronavirus disease-2019 (COVID-19), is an acute respiratory disease that causes a major impact on the world. Indonesia is one of the countries that has a high mortality rate of COVID-19. Based on the WHO and the CDC, molecular test method is the recommendation for diagnosing COVID-19. Real-time PCR test is an examination that is currently the gold standard for detecting SARS-CoV-2. There are currently many kits and reagents available to detect COVID-19, one of them is BioCoV-19 RT-PCR Kit from Biofarma that produced in Indonesia. The aim of this study was to compare basic analytical and clinical performance of BioCoV-19 RT-PCR kit with N1N2 CDC RT-PCR as the standard. In this study we used serial dilutions of SARS-CoV-2 viral RNA to establish PCR efficiency and estimate the limit of detection (LOD). Furthermore, we ran a panel of 135 clinical samples and compare the result for sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV). Finally, we used clinical samples positive for non-coronavirus respiratory viral infections for cross reactions test. PCR efficiency of BioCoV-19 RT-PCR kit was ≥96 % and the estimated LOD within a 5-fold range. The assay does not showed cross-reactivity with other respiratory viruses. We conclude that real-time BioCoV-19 RT-PCR kit assessed in this study may be used for routine diagnostics of COVID-19 in patients.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
EditorsMuhammad Nabil Mohd Warid, Muhammed Abdullah Al Sayem Khan
PublisherAmerican Institute of Physics Inc.
Edition1
ISBN (Electronic)9780735447295
DOIs
Publication statusPublished - 21 Nov 2023
Event1st Technology and Policy for Supporting Implementation of COVID-19 Response and Recovery Plan in Southeast Asia, ITTP-COVID19 2021 - Virtual, Online, Malaysia
Duration: 6 Aug 20218 Aug 2021

Publication series

NameAIP Conference Proceedings
Number1
Volume2739
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st Technology and Policy for Supporting Implementation of COVID-19 Response and Recovery Plan in Southeast Asia, ITTP-COVID19 2021
Country/TerritoryMalaysia
CityVirtual, Online
Period6/08/218/08/21

Fingerprint

Dive into the research topics of 'Comparison of Real-Time BIOCOV-19 RT-PCR Kit with N1N2 CDC RT-PCR for Detecting SARS-COV-2'. Together they form a unique fingerprint.

Cite this