TY - JOUR
T1 - Comparison of Malassezia spp. Proportions in inflammatory and noninflammatory facial acne vulgaris lesions
AU - Sutarjo, Agassi Suseno
AU - Sitohang, Irma Bernadette S.
AU - Wahid, Mardiastuti H.
AU - Widaty, Sandra
N1 - Funding Information:
The authors would like to express our sincere gratitude to the laboratory staff of the Department of Clinical Microbiology, Faculty of Medicine Universitas Indonesia for assistance in fungal culture. We would also like to thank the laboratory staff of the Department of Dermatology and Venereology for assistance in spore identification. This article was presented in the 4th International Conference and Exhibition on the Indonesian Medical Education and Research Institute (ICE on IMERI 2019), Faculty of Medicine, Universitas Indonesia. We thank the 4th ICE on IMERI committee, who had supported the peer review and manuscript preparation before submitting to the journal.
Publisher Copyright:
© 2020 The Authors.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Objective: The skin microbiome plays a role in the pathogenesis of acne vulgaris (AV). Among other species, Malassezia spp. can be found in the pilosebaceous follicle. However, its role on the pathogenesis of AV has not yet been described. The objective of this study was to identify the presence and the distribution of Malassezia spp. in facial AV lesions and also to compare the distribution of Malassezia spp. between inflammatory and non-inflammatory lesions. Methods: One hundred and twenty subjects were allocated into two groups: inflammatory lesions and non-inflammatory lesions. Samples were taken from hair follicles and examined by microscopy using 20% potassium hydroxide and Parker ink and cultured for species identification. Results: There was no difference in the spore load between the two groups (p=0.142). Malassezia spp. were isolated from 64.2% of subjects and consisted of M. dermatis (43%), M. sympodialis (18%), M. slooffiae (16%), M. japonica (5%), M. furfur (5%), M. pachydermatis (3%), and M. restricta (1%). There was a higher Malassezia spp. proportion in non-inflammatory lesions (p=0.019). The predominant species in inflammatory AV lesions was M. dermatis (45,8%), followed by M. sympodialis (17.1%), and M. slooffiae (11.4%). Conclusion: Malassezia spp. were found in facial acne lesions. M. dermatis was the predominant species found in facial AV, followed by M. sympodialis, and M. slooffiae. A higher proportion of Malassezia spp. was found in non-inflammatory lesions.
AB - Objective: The skin microbiome plays a role in the pathogenesis of acne vulgaris (AV). Among other species, Malassezia spp. can be found in the pilosebaceous follicle. However, its role on the pathogenesis of AV has not yet been described. The objective of this study was to identify the presence and the distribution of Malassezia spp. in facial AV lesions and also to compare the distribution of Malassezia spp. between inflammatory and non-inflammatory lesions. Methods: One hundred and twenty subjects were allocated into two groups: inflammatory lesions and non-inflammatory lesions. Samples were taken from hair follicles and examined by microscopy using 20% potassium hydroxide and Parker ink and cultured for species identification. Results: There was no difference in the spore load between the two groups (p=0.142). Malassezia spp. were isolated from 64.2% of subjects and consisted of M. dermatis (43%), M. sympodialis (18%), M. slooffiae (16%), M. japonica (5%), M. furfur (5%), M. pachydermatis (3%), and M. restricta (1%). There was a higher Malassezia spp. proportion in non-inflammatory lesions (p=0.019). The predominant species in inflammatory AV lesions was M. dermatis (45,8%), followed by M. sympodialis (17.1%), and M. slooffiae (11.4%). Conclusion: Malassezia spp. were found in facial acne lesions. M. dermatis was the predominant species found in facial AV, followed by M. sympodialis, and M. slooffiae. A higher proportion of Malassezia spp. was found in non-inflammatory lesions.
KW - Acne lesion
KW - Malassezia spp
KW - Skin microbiome
UR - http://www.scopus.com/inward/record.url?scp=85099654903&partnerID=8YFLogxK
U2 - 10.22159/ijap.2020.v12s3.39454
DO - 10.22159/ijap.2020.v12s3.39454
M3 - Article
AN - SCOPUS:85099654903
SN - 0975-7058
VL - 12
SP - 7
EP - 11
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - Special Issue 3
ER -