Comparison of inhibitory activity against the α-glucosidase enzymes in the extracts and fractions from leaves of the Garcinia kydia Roxburgh

Septiani Martha, Berna Elya, Muhammad Hanafi

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Abstract

Objective: Garcinia kydia Roxb. is a species of the genus Garcinia, is based chemotaxonomic has various bioactive compounds that have been isolated by a variety of pharmacological activities, one of the activities that are being developed that inhibition of α-glucosidase. However, α-glucosidase inhibitory activity in the extracts and fraction from leaves of the G. kydia Roxb. has not been reported. In this study, seeks to evaluate of α-glucosidase inhibitory activity against extracts and fractions of potentially. Methods: The α-glucosidase inhibitory activity test conducted by in vitro using the enzymatic reaction is measured of quantity with a microplate reader and identifies the compound from the active fraction with normal-phase thin layer chromatography. Results: The ethyl acetate and methanol extract have the potential to inhibit the α-glucosidase with the percent inhibition at a concentration of 500 µg/ml of 83% and 59%, respectively. The active fraction of the ethyl acetate extracts (FEA8) with percentage inhibition at concentrations of 100 µg/ml and inhibitory concentration (IC50) values of 80% and 2.79 μg/ml, respectively, and active fraction of the methanol extracts (FMT3) with percent inhibition at concentrations of 100 µg/ml and IC50 values of 71% and 8.43 μg/ml, respectively. Conclusion: G. kydia Roxb. evident has the potential to inhibit the α-glucosidase. Flavonoid and phenolic compounds that suspected of acts as α-glucosidase inhibitory activity. Thus, the research will continue the process of isolating the active compound so that it can be developed as natural therapeutic agents in the control of glucose.

Original languageEnglish
Pages (from-to)401-404
Number of pages4
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume10
Issue number7
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Antidiabetic
  • Garcinia kydia roxburgh
  • In vitro
  • α-glucosidase inhibitory activity

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