TY - JOUR
T1 - Comparison of fibroblast cell regeneration in three different concentrations of Wharton's Jelly mesenchymal stem cells conditioned medium (WJMSCs-CM)
AU - Untoro, E. G.
AU - Asrianti, D.
AU - Usman, M.
AU - Meidyawati, R.
AU - Margono, A.
N1 - Funding Information:
WJMSCs-CM in a 12.5% concentration demonstrated the maximum potential for fibroblast proliferation. Therefore, the umbilical cord tissues that remain as biomedical waste have great potential in dental pulp regeneration. However, further experiments with other methods related to dental pulp regeneration are required to analyze the potency of WJMSCs-CM. 5. Acknowledgment This research was funded by PITTA grant No. 1943/UN2.R12/HKP.05.00/2016 from the Directorate of Research and Community Engagement, Universitas Indonesia, Jakarta, Indonesia. The author also thanks Dr. Indah Julianto, Sp.KK, for support and advice during this research and Dermama Biotechnology Laboratory for providing materials.
Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2017/8/30
Y1 - 2017/8/30
N2 - Wharton's Jelly-derived mesenchymal stem cells (WJMSCs) have gained interest as an alternative source of stem cells for regenerative medicine. Although many studies have characterized Wharton's Jelly biologically, the effects of different concentrations in a cultured medium have not yet been compared. Damaged fibroblasts, the primary components of irreversible dental pulpitis, irreversibly impair the ability to regenerate and lead to the disruption of extracellular matrix. This study was performed to evaluate the potency of three WJMSCs-CM concentrations in improving serum-starved fibroblasts. Fibroblasts were cultivated in five passages, and divided into four groups. The first group (the control group) consisted of fibroblast cells that had been treated using starvation methods. The other groups (the treatment groups) were treated with various concentration of WJMSCs-CM (50%, 25% and 12.5%). Proliferative ability was evaluated using a cell count method and analyzed with a one-way ANOVA. Cultivation of serum-starved fibroblasts produced significantly higher cell counts in 12.5% WJMSCs-CM compared to the 50% group. It can be concluded that 12.5% WJMSCs-CM is the most efficient concentration for fibroblast proliferation.
AB - Wharton's Jelly-derived mesenchymal stem cells (WJMSCs) have gained interest as an alternative source of stem cells for regenerative medicine. Although many studies have characterized Wharton's Jelly biologically, the effects of different concentrations in a cultured medium have not yet been compared. Damaged fibroblasts, the primary components of irreversible dental pulpitis, irreversibly impair the ability to regenerate and lead to the disruption of extracellular matrix. This study was performed to evaluate the potency of three WJMSCs-CM concentrations in improving serum-starved fibroblasts. Fibroblasts were cultivated in five passages, and divided into four groups. The first group (the control group) consisted of fibroblast cells that had been treated using starvation methods. The other groups (the treatment groups) were treated with various concentration of WJMSCs-CM (50%, 25% and 12.5%). Proliferative ability was evaluated using a cell count method and analyzed with a one-way ANOVA. Cultivation of serum-starved fibroblasts produced significantly higher cell counts in 12.5% WJMSCs-CM compared to the 50% group. It can be concluded that 12.5% WJMSCs-CM is the most efficient concentration for fibroblast proliferation.
UR - http://www.scopus.com/inward/record.url?scp=85029581755&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/884/1/012067
DO - 10.1088/1742-6596/884/1/012067
M3 - Conference article
AN - SCOPUS:85029581755
VL - 884
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
SN - 1742-6588
IS - 1
M1 - 012067
T2 - 1st Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2017
Y2 - 15 July 2017 through 16 July 2017
ER -