TY - JOUR
T1 - Comparison of decomposition rate of hind limbs of preserved mice with ethanol-glycerin and formaldehyde of advanced fixative solution
AU - Wijaya, Amanda Natalie
AU - Margiana, Ria
AU - Kusumaningtyas, Sasanthy
AU - Furqonita, Deswaty
N1 - Funding Information:
We would like to thank Universitas Indonesia Research Grant, International Indexed Publication (PUTI) program 2020 with contract number NKB-913/UN2.RST/HKP.05.00/2020 for funding this research. This article was presented in the 5th International Conference and Exhibition on Indonesian Medical Education and Research Institute (5th ICE on IMERI), Faculty of Medicine, Universitas Indonesia. We thank the 5th ICE on IMERI committee, who have supported the peer-review and manuscript preparation before submitting it to the journal.
Funding Information:
We would like to thank Universitas Indonesia Research Grant, International Indexed Publication (PUTI) program 2020 with contract number NKB-913/UN2.RST/ HKP.05.00/2020 for funding this research. This article was presented in the 5th International Conference and Exhibition on Indonesian Medical Education and Research Institute (5th ICE on IMERI), Faculty of Medicine, Universitas Indonesia. We thank the 5th ICE on IMERI committee, who have supported the peer-review and manuscript preparation before submitting it to the journal.
Publisher Copyright:
Copyright © 2021. Anatomy & Cell Biology
PY - 2021/6
Y1 - 2021/6
N2 - Learning anatomy in medical school is still closely based on the use of cadavers. The burial of preserved cadaver poses a problem, specifically, it contaminates the soil with formalin. Many studies have been conducted to find an alternative fixative to update or modify formalin usage. One of them is ethanol-glycerin (EG), which suggests promising results. Despite that fact, there has yet to be any research comparing the decomposition rate between EG and formalin. This study is conducted to compare the rate of decomposition between the two fixative solutions, EG and 4% formalin on the hind limb of mice. The mice were first preserved using a standard primary fixative solution which is 10% formalin, following that procedure is preservation using advanced fixative solution, EG or 4% formalin. Upon completing the preservation steps, the mice were buried for 6 weeks and observed weekly. The stages of decomposition were assessed semi-quantitatively depending on its appearance. The hind limbs of mice that were fixed with EG solution managed to reach the last stage of decomposition, dry & remains, while the 4% formalin group of mice still remained in the previous stage, advanced decay. It is concluded that the mice hind limbs that have been previously preserved with EG advanced fixative solution has a faster decomposition rate compared to 4% formalin.
AB - Learning anatomy in medical school is still closely based on the use of cadavers. The burial of preserved cadaver poses a problem, specifically, it contaminates the soil with formalin. Many studies have been conducted to find an alternative fixative to update or modify formalin usage. One of them is ethanol-glycerin (EG), which suggests promising results. Despite that fact, there has yet to be any research comparing the decomposition rate between EG and formalin. This study is conducted to compare the rate of decomposition between the two fixative solutions, EG and 4% formalin on the hind limb of mice. The mice were first preserved using a standard primary fixative solution which is 10% formalin, following that procedure is preservation using advanced fixative solution, EG or 4% formalin. Upon completing the preservation steps, the mice were buried for 6 weeks and observed weekly. The stages of decomposition were assessed semi-quantitatively depending on its appearance. The hind limbs of mice that were fixed with EG solution managed to reach the last stage of decomposition, dry & remains, while the 4% formalin group of mice still remained in the previous stage, advanced decay. It is concluded that the mice hind limbs that have been previously preserved with EG advanced fixative solution has a faster decomposition rate compared to 4% formalin.
KW - Burial
KW - Glycerol
KW - Mice
KW - Preservation
UR - http://www.scopus.com/inward/record.url?scp=85109181614&partnerID=8YFLogxK
U2 - 10.5115/acb.20.314
DO - 10.5115/acb.20.314
M3 - Article
AN - SCOPUS:85109181614
SN - 2093-3665
VL - 54
SP - 225
EP - 231
JO - Anatomy and Cell Biology
JF - Anatomy and Cell Biology
IS - 2
ER -