Comparison of cytotoxicity between ethyl acetate and ethanol extract of white turmeric (kaempferia rotunda) rhizome extract against hela cervical cancer cell activity

Surya Dwira, T. P. Ariska, Fadilah Fadilah, Norma Nur Azizah, Linda Erlina

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: The aim of this study is to compare between ethanol and ethyl acetate rhizome extract of K.rotunda against HeLa cervical cancer cell in vitro. Material and Methods: Methods used in this research are test the chemical compound of extracts using Thin Layer Chromatography (TLC) and phytochemical screening test, also cytotoxicity test using MTT assay. Result: Ethyl acetate extract contains flavonoid, alkaloid, tannin, and triterpenoid, while ethanol extract have flavonoid, triterpenoid, and alkaloid. In addition, ethanol extract has strong cytotoxic activity (IC50 = 16,939 μg/ml) while ethyl acetate extract has moderate cytotoxic activity (IC50 = 127,9 μg/ml). Each of extracts showed significant results (p ≤ 0,05) although when compared between concentrations there are several concentrations that are not significant and also small coefficient of determinant values caused by various confounding factors. Conclusion: The ethanol extract of K.rotunda rhizome extract has the higher cytotoxicity activity compared to ethyl acetate extract of K.rotunda rhizome extract against HeLa cervical cancer cell.

Original languageEnglish
Pages (from-to)1297-1302
Number of pages6
JournalPharmacognosy Journal
Volume12
Issue number6
DOIs
Publication statusPublished - Dec 2020

Keywords

  • Anti cervical cancer
  • HeLa
  • In vitro
  • Kaempferia rotunda

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