TY - JOUR
T1 - Comparison of Antibacterial Activity in Ethanol Extract and Essential Oil of Citrus sinensis (L.) Peels Obtained by Sohxlet and Distillation Methods
AU - Kusrini, E.
AU - Mawarni, D. P.
AU - Mamat, M.
AU - Prasetyanto, E. A.
AU - Usman, A.
N1 - Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/10/31
Y1 - 2018/10/31
N2 - Ethanol extract and essential oil of Citrus sinensis (L.) peels have been characterized and compared for its antibacterial activity. Ethanol extract of C. sinensis (L.) peels was obtained by Soxhlet extraction method in ethanol with purity of 96% and the essential oil was obtained by distillation method for 5 hours at 100°C. The antibacterial activities of the extracts were investigated against Staphylococcus aureus. The experimental results showed that the ethanol extract yielded 29.50% (w/w), with total flavonoid compounds of 4.74 mg/g and polyphenolic of 20.54 mg/g, while the water extract (essential oil) yielded 6.80% (w/w) with total polyphenolic compounds of 0.65 mg/g. The flavonoid compound was not observed in the essential oil obtained from distillation method. The bioactive compounds in the ethanol extract obtained by Soxhlet extraction are larger than those found in the essential oil obtained by distillation method. The ethanol extract showed stronger growth inhibitory effect of Staphylococcus aureus with zone inhibition diameter of 18 mm, whereas the essential oil and the commercial d-limonene extract had similar zone inhibition diameter of 15 mm.
AB - Ethanol extract and essential oil of Citrus sinensis (L.) peels have been characterized and compared for its antibacterial activity. Ethanol extract of C. sinensis (L.) peels was obtained by Soxhlet extraction method in ethanol with purity of 96% and the essential oil was obtained by distillation method for 5 hours at 100°C. The antibacterial activities of the extracts were investigated against Staphylococcus aureus. The experimental results showed that the ethanol extract yielded 29.50% (w/w), with total flavonoid compounds of 4.74 mg/g and polyphenolic of 20.54 mg/g, while the water extract (essential oil) yielded 6.80% (w/w) with total polyphenolic compounds of 0.65 mg/g. The flavonoid compound was not observed in the essential oil obtained from distillation method. The bioactive compounds in the ethanol extract obtained by Soxhlet extraction are larger than those found in the essential oil obtained by distillation method. The ethanol extract showed stronger growth inhibitory effect of Staphylococcus aureus with zone inhibition diameter of 18 mm, whereas the essential oil and the commercial d-limonene extract had similar zone inhibition diameter of 15 mm.
UR - http://www.scopus.com/inward/record.url?scp=85056695399&partnerID=8YFLogxK
U2 - 10.1088/1757-899X/440/1/012028
DO - 10.1088/1757-899X/440/1/012028
M3 - Conference article
AN - SCOPUS:85056695399
SN - 1757-8981
VL - 440
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
IS - 1
M1 - 012028
T2 - 1st International Fundamentum Science Symposium 2018
Y2 - 25 June 2018 through 26 June 2018
ER -