Comparison of AgNP antibacterial effects with resterilization autoclave technique in used TADs orthodontics

Repeatedly autoclaved TADs have shown surface alterations which might lead to instability, whereas there is possibility of reuse TADs for same patient to reduce costs. Porphyromonas gingivalis (PG), the most common type of bacteria found in peri-implantitis, causes failure of TADs insertion. Antibacterial properties of silver nanoparticles (AgNPs) has commonly used in orthodontic. However, no study has described the antibacterial effects of AgNPs to PG colonies on used TADs surfaces. To evaluate the antimicrobial effects of AgNPs solution compared with autoclaving on the number of PG colonies in used TADs. 20 new TADs were separated into 2 groups: P1 (AgNPs solution) and P2 (autoclave). The PG antibacterial activity was evaluated by comparing the number of PG colonies on Brusella agar plates using digital electronic colony counter (103 CFU/mL) on T0 (after immersed in a plaque-forming solution containing PG ATCC 33277 and cultured under anaerobic conditions for 24 h) and T1 (after resterilized using 2 different methods and cultured under anaerobic conditions for 24 h). No PG colonies survived in both groups on T1. AgNPs solution was found to have antibacterial activity against PG equivalent to autoclaving.