Comparison between High Performance Thin Layer Chromatography and High Performance Liquid Chromatography methods for determination of rubraxanthone in the stem bark extract of Garcinia cowa Roxb

Meri Susanti, Sanusi Ibrahim, Yahdiana Harahap, Dachriyanus

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: To develop simple, rapid, accurate methods for determination of rubraxanthone in the stem bark extract of Garcinia cowa using High Performance Thin Layer Chromatography (HPTLC) and High Performance Liquid Chromatography (HPLC). Methods: The HPTLC method was performed on aluminum plate precoated with silica gel 60 F254 using Chloroform: Ethyl acetate: Methanol: Formic acid (88:2:2:8) as a developing system. Quantification was achieved using densitometric measurements at 243 nm. The HPLC method involved a 5 μm C18 column and an isocratic solvent using 0.4% formic acid: methanol (12:88) with a flow rate 1 mL minute-1. Quantitation was also achieved with ultraviolet detection at 243 nm based on peak area. All necessary validation tests for both methods were done for their comparison. The results obtained by these two different quantification methods were compared by Tukey's-test. Results: Both assays provided good linearity, accuracy, precision, specificity and limits of detection and quantitation for determination of rubraxanthone in The Stem Bark extract of G. cowa. Conclusion: Both methods revealed reasonable validation parameters concerning linearity, accuracy, precision, specificity and limits of detection and quantitation. A statistical comparison of the quantitative analysis of rubraxanthone in extract did not show any statistically significant difference between two analysis methods. As both methods were found to be equal, they therefore can be used for the analysis of rubraxanthone in the Stem Bark extract of G. cowa.

Original languageEnglish
Pages (from-to)S42-S47
JournalPharmacognosy Journal
Volume10
Issue number6
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Garcinia cowa Roxb
  • High performance liquid chromatography
  • High performance thin layer chromatography
  • Rubraxanthone

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