TY - JOUR
T1 - Comparative in vitro activity of bacteriophage endolysin HY-133 against Staphylococcus aureus attached to vascular graft surface
AU - Idelevich, Evgeny A.
AU - Knaack, Dennis
AU - Nugroho, Nyityasmono Tri
AU - Peters, Georg
AU - Bisdas, Theodosios
AU - Molinaro, Sonja
AU - Torsello, Giovanni B.
AU - Becker, Karsten
AU - Herten, Monika
N1 - Funding Information:
This research was supported by the fund: “Innovative Medical Research” from Medical School of the University of Münster (ID 111317) and partly by the BMBF-DZIF (German Center for Infection Research, TTU 08.807 HAARBI, 8037808809 and 8037808907). Vascular graft material was provided by B. Braun, Melsungen, Germany (Uni-Graft K DV). ® Acknowledgements
Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Novel strategies are needed for combating Staphylococcus aureus biofilm in vascular graft infections. We investigated the in vitro activity of bacteriophage endolysin HY-133, daptomycin and rifampin against S. aureus attached to vascular graft surface. Daptomycin showed rapid bactericidal effect on surface-associated S. aureus, while the activity of HY-133 on graft surface-adherent cells was moderate and rifampin did not achieve bactericidal effect. Even in the highest concentrations, all antimicrobials used failed in a complete eradication of the surface-adherent bacteria.
AB - Novel strategies are needed for combating Staphylococcus aureus biofilm in vascular graft infections. We investigated the in vitro activity of bacteriophage endolysin HY-133, daptomycin and rifampin against S. aureus attached to vascular graft surface. Daptomycin showed rapid bactericidal effect on surface-associated S. aureus, while the activity of HY-133 on graft surface-adherent cells was moderate and rifampin did not achieve bactericidal effect. Even in the highest concentrations, all antimicrobials used failed in a complete eradication of the surface-adherent bacteria.
KW - Antibiotics
KW - Bacteriophage endolysin
KW - Biofilm
KW - HY-133
KW - Resistance
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85074014986&partnerID=8YFLogxK
U2 - 10.1007/s00430-019-00638-1
DO - 10.1007/s00430-019-00638-1
M3 - Article
C2 - 31624909
AN - SCOPUS:85074014986
SN - 0300-8584
VL - 209
SP - 51
EP - 57
JO - Medical Microbiology and Immunology
JF - Medical Microbiology and Immunology
IS - 1
ER -