TY - JOUR
T1 - Combined enzymatic and ultrasound-assisted aqueous two-phase extraction of antidiabetic flavonoid compounds from Strobilanthes crispus leaves
AU - Arbianti, Rita
AU - Angelina,
AU - Suryapranata, Bryan
AU - Latifah, Linatri Purwati
AU - Putri, Najah Fadilah
AU - Utami, Tania Surya
AU - Muharam, Yuswan
AU - Slamet, null
N1 - Publisher Copyright:
© 2023 The Author(s).
PY - 2023
Y1 - 2023
N2 - A novel green extraction method combining enzymatic and ultrasound-assisted aqueous two-phase extractions was employed to enhance and purify the flavonoid extract from Strobilanthes crispus leaves. Cellulase was used in the pretreatment, and ethanol-(NH4)2SO4 was selected as the solvent. A concentration of 7% (w/w) cellulase and a duration of 2 h were the optimal conditions for pretreatment. The optimal conditions for ultrasound-assisted aqueous two-phase extraction were 33% (w/w) ethanol and 14% (w/w) (NH4)2SO4as they produced a yield (77.81%), partition coefficient (31.17), extraction efficiency (98.04%), and a high total flavonoid content (0.3666 mg QE/g dry leaf powder). Six compounds from the leaf extract were identified through liquid chromatography-mass spectrometry/mass spectrometry-quadrupole-time of flight (LCMS/MS-Q-TOF) analysis. The crude extract and three compounds in it (kaempferol, graveobioside A, and genistein) showed an antidiabetic activity with IC50 values of 390.35, 201.87, 292.73, and 431.82 mg/mL, respectively. These values are comparable to the standard drug acarbose.
AB - A novel green extraction method combining enzymatic and ultrasound-assisted aqueous two-phase extractions was employed to enhance and purify the flavonoid extract from Strobilanthes crispus leaves. Cellulase was used in the pretreatment, and ethanol-(NH4)2SO4 was selected as the solvent. A concentration of 7% (w/w) cellulase and a duration of 2 h were the optimal conditions for pretreatment. The optimal conditions for ultrasound-assisted aqueous two-phase extraction were 33% (w/w) ethanol and 14% (w/w) (NH4)2SO4as they produced a yield (77.81%), partition coefficient (31.17), extraction efficiency (98.04%), and a high total flavonoid content (0.3666 mg QE/g dry leaf powder). Six compounds from the leaf extract were identified through liquid chromatography-mass spectrometry/mass spectrometry-quadrupole-time of flight (LCMS/MS-Q-TOF) analysis. The crude extract and three compounds in it (kaempferol, graveobioside A, and genistein) showed an antidiabetic activity with IC50 values of 390.35, 201.87, 292.73, and 431.82 mg/mL, respectively. These values are comparable to the standard drug acarbose.
KW - antidiabetic
KW - aqueous two-phase
KW - enzymatic
KW - S. crispus
KW - Ultrasound
UR - http://www.scopus.com/inward/record.url?scp=85183717216&partnerID=8YFLogxK
U2 - 10.21924/cst.8.2.2023.1214
DO - 10.21924/cst.8.2.2023.1214
M3 - Article
AN - SCOPUS:85183717216
SN - 2502-9258
VL - 8
SP - 113
EP - 123
JO - Communications in Science and Technology
JF - Communications in Science and Technology
IS - 2
ER -