Column chromatography isolation of nicotine from tobacco leaf extract (Nicotiana tabaccum L.)

Raden Muhammad Fathi, Ahmad Fauzantoro, Siti Fauziyah Rahman, Misri

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Restrictions on the use of dried tobacco leaf for cigarette production must be accompanied by the development of non-cigarette alternative products that are made from tobacco leaves. One of the alternative that can be done is to use the nicotine compound in tobacco leaf extract as medical product, such as Parkinson's medication or to be used as active substance in biopesticide. Nicotine was isolated using column chromatography method with the variation of mobile phase mixture ratio (petroleum ether and ethanol), started from 8:2, 6:4, 4:6, 2:8, to 0:10. All of the chromatographic fraction from each mobile phase's ratio was then tested qualitatively using thin layer chromatography (TLC) and also quantitatively using HPLC instrument. The column chromatography process could isolate 4.006% of nicotine compound from 4.19% tobacco leaf extract's nicotine. It is also known that ethanol is a good solution to be used as chromatography's mobile phase for nicotine isolation from tobacco leaf extract.

Original languageEnglish
Title of host publication2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2017
EditorsRadon Dhelika, Yudan Whulanza, Ghiska Ramahdita, Praswasti P.D.K. Wulan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735416253
DOIs
Publication statusPublished - 13 Feb 2018
Event2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017 - Bali, Indonesia
Duration: 25 Jul 201726 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume1933
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017
CountryIndonesia
CityBali
Period25/07/1726/07/17

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