Coliform detection in Indonesian Ragi Tapai as an indicator of unhygienic processes using chromogenic media

Q. G. Fadhilah, I. Santoso, Y. Yasman, A. E. Maryanto, S. Sitaresmi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Coliform bacteria can be used as an indicator for unhygienic conditions related to food processes. Detection of coliform bacteria in Indonesian ragi tapai has been performed using chromogenic media. The chromogenic media were chosen because the reaction is faster and more reliable compared to the conventional media. Fifteen samples of ragi tapai from West Java, Central Java and outside Java region in Indonesia were analyzed for the presence of coliform. The analysis was carried out in Readycult Coliforms (RC) medium using the Presence/Absence test. Incubation temperature was 37 °C for total coliform and 44.5 °C for faecal coliform. The presence of Escherichia coli was confirmed by indole ring formation, fluorescence of medium when exposed to UV light and formation of blue to violet colonies on Chromocult Coliform Agar Enhanced Selectivity (CCA-ES) medium. The positive results showed that from 15 samples, 12 samples (80 %) tested positive for coliform and 3 samples (20 %) tested negative in RC medium. One of 12 samples tested positive for faecal coliform.

Original languageEnglish
Title of host publicationProceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018
EditorsTerry Mart, Djoko Triyono, Ivandini T. Anggraningrum
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419155
DOIs
Publication statusPublished - 4 Nov 2019
Event4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018 - Depok, Indonesia
Duration: 30 Oct 201831 Oct 2018

Publication series

NameAIP Conference Proceedings
Volume2168
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium on Current Progress in Mathematics and Sciences 2018, ISCPMS 2018
CountryIndonesia
CityDepok
Period30/10/1831/10/18

Keywords

  • Chromogenic media
  • coliform
  • presence/absence test
  • ragi tapai

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    Fadhilah, Q. G., Santoso, I., Yasman, Y., Maryanto, A. E., & Sitaresmi, S. (2019). Coliform detection in Indonesian Ragi Tapai as an indicator of unhygienic processes using chromogenic media. In T. Mart, D. Triyono, & I. T. Anggraningrum (Eds.), Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2018 [020081] (AIP Conference Proceedings; Vol. 2168). American Institute of Physics Inc.. https://doi.org/10.1063/1.5132508