TY - JOUR
T1 - Clinical characteristics influence cultivable-bacteria composition in the meconium of Indonesian neonates
AU - Jonathan, Kristien Juni Thandwi
AU - Ong, Grasella
AU - Prasetyaningsih, Firsty Amanah
AU - Amandito, Radhian
AU - Rohsiswatmo, Rinawati
AU - Malik, Amarila
N1 - Funding Information:
This work was supported by Universitas Indonesia Research Grant for Q1 and Q2 International Publication year 2019 (No. NKB-0194/UN2.R3.1/HKP.05.00/5522019) to AM and RR. This research is also partially funded by the Indonesian Ministry of Research and Technology / National Agency for Research and Innovation , and the Indonesian Ministry of Education and Culture under the World Class University (WCU) Program managed by Institut Teknologi Bandung (ITB) to AM.
Publisher Copyright:
© 2020 The Author(s)
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/12
Y1 - 2020/12
N2 - Background: Microbial colonization of a neonate's gastrointestinal tract has significant perinatal and lifetime health consequences. However, information regarding the profile of meconium microbiota in neonates and the influence of clinical parameters are lacking in the Indonesian population. This study aimed to preliminary investigate the profile of cultivable bacterial diversity of meconium isolated from neonates born at Cipto Mangunkusumo Hospital (CMH), Jakarta. The cultivable bacteria were isolated from meconium samples and were then processed for cultivation and molecular identification. Results: Fourteen neonates were enrolled as described, i.e., seven hyperbilirubinemia (Hyp) and seven non-Hyp with ten neonates delivered by cesarean section (CS) and four others by vaginal route (VR), and with five exclusive breastfeeding (Ebf), four formula milk, and five combinations. Microbiological identification, molecular 16S rDNA PCR-Sanger sequencing, and PCA analysis of cultivable bacteria isolated from meconium showed Firmicutes' predominance (84.41%), with an abundant population of Staphylococcus, which consist of S. hominis, S. epidermidis, and S. haemolyticus. The influence of mode of delivery showed a lower diversity than the CS populates the VR, but their composition was similar. Concurrently, between feeding patterns, the genera profile did not show much difference; in the non-Ebf group, the total amount of Staphylococcus and Bacillus showed a higher amount but a less diverse. Interestingly, the non-Hyp group showed more abundant and diverse Staphylococcus than that of the Hyp group. In contrast, neonates diagnosed with NEC and proven sepsis showed the same pattern of Staphylococcus domination. Conclusion: Staphylococcus predominated the composition of cultivable bacteria in neonates meconium. Due to the small sample size, only the hyperbilirubinemia parameter significantly influenced the profile, i.e., Staphylococcus's proportion (p = 0.037).
AB - Background: Microbial colonization of a neonate's gastrointestinal tract has significant perinatal and lifetime health consequences. However, information regarding the profile of meconium microbiota in neonates and the influence of clinical parameters are lacking in the Indonesian population. This study aimed to preliminary investigate the profile of cultivable bacterial diversity of meconium isolated from neonates born at Cipto Mangunkusumo Hospital (CMH), Jakarta. The cultivable bacteria were isolated from meconium samples and were then processed for cultivation and molecular identification. Results: Fourteen neonates were enrolled as described, i.e., seven hyperbilirubinemia (Hyp) and seven non-Hyp with ten neonates delivered by cesarean section (CS) and four others by vaginal route (VR), and with five exclusive breastfeeding (Ebf), four formula milk, and five combinations. Microbiological identification, molecular 16S rDNA PCR-Sanger sequencing, and PCA analysis of cultivable bacteria isolated from meconium showed Firmicutes' predominance (84.41%), with an abundant population of Staphylococcus, which consist of S. hominis, S. epidermidis, and S. haemolyticus. The influence of mode of delivery showed a lower diversity than the CS populates the VR, but their composition was similar. Concurrently, between feeding patterns, the genera profile did not show much difference; in the non-Ebf group, the total amount of Staphylococcus and Bacillus showed a higher amount but a less diverse. Interestingly, the non-Hyp group showed more abundant and diverse Staphylococcus than that of the Hyp group. In contrast, neonates diagnosed with NEC and proven sepsis showed the same pattern of Staphylococcus domination. Conclusion: Staphylococcus predominated the composition of cultivable bacteria in neonates meconium. Due to the small sample size, only the hyperbilirubinemia parameter significantly influenced the profile, i.e., Staphylococcus's proportion (p = 0.037).
KW - Clinical characteristic
KW - Clinical outcomes
KW - Clinical research
KW - Digestive system
KW - Feeding patterns
KW - Gastrointestinal system
KW - Health sciences
KW - Hyperbilirubinemia
KW - Indonesia
KW - Meconium
KW - Microbiology
KW - Microbiota
KW - Mode of delivery
KW - Neonates
KW - Pediatrics
UR - http://www.scopus.com/inward/record.url?scp=85096987960&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2020.e05576
DO - 10.1016/j.heliyon.2020.e05576
M3 - Article
AN - SCOPUS:85096987960
SN - 2405-8440
VL - 6
JO - Heliyon
JF - Heliyon
IS - 12
M1 - e05576
ER -