TY - GEN
T1 - Characterization of potential biocontrol agent Bacillus sp.
AU - Santoso, I.
AU - Fadhilah, Q. G.
AU - Maryanto, A. E.
AU - Yasman, null
N1 - Funding Information:
The research was supported by Grant PUTI Prosiding 2020 from Universitas Indonesia to Drs. Iman Santoso, M.Phil. (No. NKB-939/UN2.RST/HKP.05.00/2020).
Publisher Copyright:
© 2021 Author(s).
PY - 2021/7/23
Y1 - 2021/7/23
N2 - Three potential Bacillus sp. strain, KRG, KRT, and LDR from cocopeat was known to have antagonistic activity against fungal pathogen. The antagonistic activity represented the potency of three isolates as biocontrol agent. In this study, the growth and extracellular enzyme of three isolates will be characterized and also the selected bacterium was identified based on 16S rRNA. The growth of Bacillus sp. strains were characterized in Potato Dextrose Broth (PDB) with various pH medium and temperature incubation. The three isolates can growth at 30-45 °C, meanwhile KRG can grew up to 50 °C. The optimum temperature growth of LDR was 30-45 °C. All of isolates can grow in medium with pH 5-8 with optimal growth for LDR in pH 7-8. Extracellular enzyme assay showed all isolates can produce amylase, lipase, protease, and gelatinase. Molecular identification showed that selected strain LDR is closed to Bacillus siamensis and has 99.52 % similarity with Bacillus siamensis KCTC 13613 strain PD-A10.
AB - Three potential Bacillus sp. strain, KRG, KRT, and LDR from cocopeat was known to have antagonistic activity against fungal pathogen. The antagonistic activity represented the potency of three isolates as biocontrol agent. In this study, the growth and extracellular enzyme of three isolates will be characterized and also the selected bacterium was identified based on 16S rRNA. The growth of Bacillus sp. strains were characterized in Potato Dextrose Broth (PDB) with various pH medium and temperature incubation. The three isolates can growth at 30-45 °C, meanwhile KRG can grew up to 50 °C. The optimum temperature growth of LDR was 30-45 °C. All of isolates can grow in medium with pH 5-8 with optimal growth for LDR in pH 7-8. Extracellular enzyme assay showed all isolates can produce amylase, lipase, protease, and gelatinase. Molecular identification showed that selected strain LDR is closed to Bacillus siamensis and has 99.52 % similarity with Bacillus siamensis KCTC 13613 strain PD-A10.
KW - Bacillus siamensis LDR
KW - extracellular enzyme
KW - molecular identification
KW - physiological characterization
UR - http://www.scopus.com/inward/record.url?scp=85112066164&partnerID=8YFLogxK
U2 - 10.1063/5.0058783
DO - 10.1063/5.0058783
M3 - Conference contribution
AN - SCOPUS:85112066164
T3 - AIP Conference Proceedings
BT - Proceedings of the 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020
A2 - Ivandini, Tribidasari A.
A2 - Churchill, David G.
A2 - Lee, Youngil
A2 - Alias, Yatimah Binti
A2 - Margules, Chris
PB - American Institute of Physics Inc.
T2 - 6th International Symposium on Current Progress in Mathematics and Sciences 2020, ISCPMS 2020
Y2 - 27 October 2020 through 28 October 2020
ER -
