Characterization of iron binding peptides from soybean hydrolysates produced by thermal and enzimatyc process

R. Giarni, E. Saepudin, I. Susanti, N. Laily

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Anemia due to iron deficiency is a global problem that requires serious awareness. Iron deficiency can be caused by the lack of iron intake, and low absorption of iron. It is known that peptides obtained from protein hydrolysates can generally act as iron chelating. Peptides can increase iron solubility, bioavailability, absorption and stability. BPPT (Agency for the Assessment and Application of Technology) developed soybean hydrolysates, which was obtained by thermal and enzymatic hydrolysis. Soybean hydrolysates had been tested for iron binding using in vitro test. The aim of this study was to characterize peptides from soybean hydrolysates which had iron binding activity. This research was conducted with two stages of separation, which were using 10 kD Molecular Weight Cut Off ultrafiltration membrane and gel filtration chromatography used the Superdex 30 column (GE Healthcare, USA) was coupled with Aktaprime. Furthermore, to find out the character of the peptide which acts as a promoter of iron binding, analysis of protein molecular weights using SDS-PAGE and analysis of composition amino acids using HPLC were performed. The results showed that the peptides from soybean hydrolysate which act as iron binder had a molecular weight of 10 kDa. Composition of amino acids in the fraction were dominated by arginine, aspartic acid and glutamic acid.

Original languageEnglish
Title of host publicationProceedings of the 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
EditorsTerry Mart, Djoko Triyono, Tribidasari Anggraningrum Ivandini
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420014
DOIs
Publication statusPublished - 1 Jun 2020
Event5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019 - Depok, Indonesia
Duration: 9 Jul 201910 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2242
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
CountryIndonesia
CityDepok
Period9/07/1910/07/19

Keywords

  • amino acid
  • anemia
  • iron binding
  • peptide
  • Soybean

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