TY - JOUR
T1 - Characterization of Hypoxia-Preconditioned Umbilical Cord Mesenchymal Stem Cells Derived From Macaca fascicularis
AU - Rinendyaputri, Ratih
AU - Dany, Frans
AU - Mariya, Sela Septima
AU - Panjaitan, Novaria Sari Dewi
AU - Nikmah, Uly Alfi
AU - Nareswara, Hieronimus Adiyoga
AU - Dumingan, Alvian
AU - Sonny, Sonny
AU - Sanjaya, Rio Handi
AU - Malik, Amarila
AU - Tazkia, Rahardianty Hanum
AU - Irnidiyanti, Yulia
AU - Idrus, Hasta Handayani
AU - Ayuningtyas, Wireni
AU - Noverina, Rachmawati
AU - Huda, Fathul
AU - Faried, Ahmad
N1 - Publisher Copyright:
© 2026 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2026/2
Y1 - 2026/2
N2 - UC-MSCs appear to be a viable option for stem cell-based treatment. Cynomolgus monkeys, with their psychological and genetic parallels to humans, are essential models for medical study and development. Hypoxia is a basic characteristic of angiogenesis, glucose metabolism, and cell proliferation and survival in both healthy and pathological individuals. This study aimed to develop and characterize UC-MSCs requirements derived from the cynomolgus monkey. We have successfully developed UC-MSCs derived from Mf under hypoxic conditions. Mf UC-MSCs was cultured from the WJ area. Our Mf UC-MSCs have developed into adipocytes, osteocytes, and chondrocytes. The protein of the biomarker MSC was expressed in our hypoxic precondition Mf UC-MSCs and the mRNA expression levels of ERK1/2, AKT1, and HIF-1 alpha increased in the hypoxic condition compared to the normoxic and control groups. The JNK and NFκB genes were decreased in the hypoxic group. Interestingly, our hypoxic Mf UC-MSCs suppressed IL-6 expression. These findings show that preconditioned Mf UC-MSCs produce anti-inflammatory properties, making them potential candidates for regenerative medicine. Their response to hypoxia is crucial for developing targeted therapies to enhance regenerative capabilities in conditions such as ischemic injury and neurodegeneration.
AB - UC-MSCs appear to be a viable option for stem cell-based treatment. Cynomolgus monkeys, with their psychological and genetic parallels to humans, are essential models for medical study and development. Hypoxia is a basic characteristic of angiogenesis, glucose metabolism, and cell proliferation and survival in both healthy and pathological individuals. This study aimed to develop and characterize UC-MSCs requirements derived from the cynomolgus monkey. We have successfully developed UC-MSCs derived from Mf under hypoxic conditions. Mf UC-MSCs was cultured from the WJ area. Our Mf UC-MSCs have developed into adipocytes, osteocytes, and chondrocytes. The protein of the biomarker MSC was expressed in our hypoxic precondition Mf UC-MSCs and the mRNA expression levels of ERK1/2, AKT1, and HIF-1 alpha increased in the hypoxic condition compared to the normoxic and control groups. The JNK and NFκB genes were decreased in the hypoxic group. Interestingly, our hypoxic Mf UC-MSCs suppressed IL-6 expression. These findings show that preconditioned Mf UC-MSCs produce anti-inflammatory properties, making them potential candidates for regenerative medicine. Their response to hypoxia is crucial for developing targeted therapies to enhance regenerative capabilities in conditions such as ischemic injury and neurodegeneration.
KW - cynomolgus monkey
KW - hypoxic
KW - IL-6
KW - Mf UC-MSCs
UR - https://www.scopus.com/pages/publications/105026961494
U2 - 10.1111/jmp.70056
DO - 10.1111/jmp.70056
M3 - Article
C2 - 41501980
AN - SCOPUS:105026961494
SN - 0047-2565
VL - 55
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
IS - 1
M1 - e70056
ER -