TY - JOUR
T1 - Characterization of dengue virus serotype 4 infection in Jakarta, indonesia
AU - Dewi, Beti Ernawati
AU - Naiggolan, Leonard
AU - Putri, Dwi Hilda
AU - Rachmayanti, Novia
AU - Albar, Sarah
AU - Indriastuti, Nadia Tita
AU - Sjamsuridzal, Wellyzar
AU - Sudiro, Tjahjani Mirawati
PY - 2014
Y1 - 2014
N2 - Dengue hemorrhagic fever has become a worldwide health issue. Heterologous infection by different serotypes may lead to severe forms of dengue infection and even death. In a cohort study in Jakarta from 2009 to 2010 with inclusion criteria of adults with fever of less than 48 hours, 72% were confirmed dengue infection from a total of suspected 190 dengue patients. Using RT-PCR, 16 patients were infected with DENV-4 with or without co-infection with other serotype(s). Dengue fever (DF) patients (73%) were infected with DENV-4 alone, while mixed infections were present in more severe clinical manifestations of DHF grade I and II. The nucleotide sequences of envelope (E) protein gene of 3 isolates of DENV-4 showed that they belonged to genotype II. There were 50 nucleotide substitutions, but only 3 amino acid changes were found at positions 130, 233 and 455, present in E protein isolated from DHF grade II patient.
AB - Dengue hemorrhagic fever has become a worldwide health issue. Heterologous infection by different serotypes may lead to severe forms of dengue infection and even death. In a cohort study in Jakarta from 2009 to 2010 with inclusion criteria of adults with fever of less than 48 hours, 72% were confirmed dengue infection from a total of suspected 190 dengue patients. Using RT-PCR, 16 patients were infected with DENV-4 with or without co-infection with other serotype(s). Dengue fever (DF) patients (73%) were infected with DENV-4 alone, while mixed infections were present in more severe clinical manifestations of DHF grade I and II. The nucleotide sequences of envelope (E) protein gene of 3 isolates of DENV-4 showed that they belonged to genotype II. There were 50 nucleotide substitutions, but only 3 amino acid changes were found at positions 130, 233 and 455, present in E protein isolated from DHF grade II patient.
KW - Dengue virus serotype 4
KW - Genotype II
KW - Indonesia
KW - Serotype mixed infection
UR - http://www.scopus.com/inward/record.url?scp=84899559827&partnerID=8YFLogxK
M3 - Article
C2 - 24964654
AN - SCOPUS:84899559827
SN - 0125-1562
VL - 45
SP - 53
EP - 61
JO - Southeast Asian Journal of Tropical Medicine and Public Health
JF - Southeast Asian Journal of Tropical Medicine and Public Health
IS - 1
ER -