Characterization of colon cancer cells from a patient at Ciptomangun Kusumo National Hospital using CD44, NANOG and OCT4 gene

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.

Original languageEnglish
Title of host publicationProceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019
Subtitle of host publicationTowards Innovative Research and Cross-Disciplinary Collaborations
EditorsNaji Arafat Mahat, Roswanira Ab. Wahab, Fahrul Zaman Huyop, Aemi Syazwani Abdul Keyon, Nursyafreena Binti Attan, Sheela Chandren, Ida Bagus Wayan Gunam
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419001
DOIs
Publication statusPublished - 6 Sep 2019
Event2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019 - Bali, Indonesia
Duration: 11 Apr 201912 Apr 2019

Publication series

NameAIP Conference Proceedings
Volume2155
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019
CountryIndonesia
CityBali
Period11/04/1912/04/19

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  • Cite this

    Abdullah, M., Bela, B., Syam, A. F., Simadibrata, M., Meilany, S., Annisa, F., Amirulloh, D., Makmun, D., & Rani, A. A. (2019). Characterization of colon cancer cells from a patient at Ciptomangun Kusumo National Hospital using CD44, NANOG and OCT4 gene. In N. A. Mahat, R. A. Wahab, F. Z. Huyop, A. S. A. Keyon, N. B. Attan, S. Chandren, & I. B. W. Gunam (Eds.), Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019: Towards Innovative Research and Cross-Disciplinary Collaborations [020041] (AIP Conference Proceedings; Vol. 2155). American Institute of Physics Inc.. https://doi.org/10.1063/1.5125545