TY - GEN
T1 - Characterization of colon cancer cells from a patient at Ciptomangun Kusumo National Hospital using CD44, NANOG and OCT4 gene
AU - Abdullah, Murdani
AU - Bela, Budiman
AU - Syam, Ari Fahrial
AU - Simadibrata, Marcellus
AU - Meilany, Sofy
AU - Annisa, Firda
AU - Amirulloh, Dian
AU - Makmun, Dadang
AU - Rani, Abdul Aziz
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/9/6
Y1 - 2019/9/6
N2 - CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.
AB - CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.
UR - http://www.scopus.com/inward/record.url?scp=85072615356&partnerID=8YFLogxK
U2 - 10.1063/1.5125545
DO - 10.1063/1.5125545
M3 - Conference contribution
AN - SCOPUS:85072615356
T3 - AIP Conference Proceedings
BT - Proceedings of the 2nd International Conference on Biosciences and Medical Engineering, ICBME 2019
A2 - Mahat, Naji Arafat
A2 - Wahab, Roswanira Ab.
A2 - Huyop, Fahrul Zaman
A2 - Keyon, Aemi Syazwani Abdul
A2 - Attan, Nursyafreena Binti
A2 - Chandren, Sheela
A2 - Gunam, Ida Bagus Wayan
PB - American Institute of Physics Inc.
T2 - 2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019
Y2 - 11 April 2019 through 12 April 2019
ER -
