TY - JOUR
T1 - Characterization of a flavinogenic mutant of methanol yeast Candida boidinii and its extracellular secretion of riboflavin
AU - Suryadi, Herman
AU - Yoshida, Nobuyuki
AU - Yamada-Onodera, Keiko
AU - Katsuragi, Tohoru
AU - Tani, Yoshiki
N1 - Funding Information:
We thank Mrs. Sachiko Iida of Iida Foods Co., Yao, Osaka, and the Ministry of Education, Science, Sports, and Culture, Japan for financial support of H. S.
PY - 2000/7
Y1 - 2000/7
N2 - A flavinogenic mutant was derived from Candida boidinii by mutagenesis. The mutant was smaller than the wild type, did not grow on a minimal medium, and required L-tryptophan, L-leucine, inositol, and nicotinate for growth. The mutant was defective in the oxidative pentose phosphate pathway, lacking glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. The specific activities of the transaldolase and transketolase of the mutant were higher than those of the wild type. These high activities might direct the flux of the carbon source to the nonoxidative pathway with formation of a large amount of pentose phosphates, increasing riboflavin synthesis. Under microaerobic conditions at 25°C, 90 mg/l riboflavin was obtained.
AB - A flavinogenic mutant was derived from Candida boidinii by mutagenesis. The mutant was smaller than the wild type, did not grow on a minimal medium, and required L-tryptophan, L-leucine, inositol, and nicotinate for growth. The mutant was defective in the oxidative pentose phosphate pathway, lacking glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. The specific activities of the transaldolase and transketolase of the mutant were higher than those of the wild type. These high activities might direct the flux of the carbon source to the nonoxidative pathway with formation of a large amount of pentose phosphates, increasing riboflavin synthesis. Under microaerobic conditions at 25°C, 90 mg/l riboflavin was obtained.
KW - Candida boidinii
KW - Mutagenesis
KW - Pentose phosphate
KW - Riboflavin
UR - http://www.scopus.com/inward/record.url?scp=0034233142&partnerID=8YFLogxK
U2 - 10.1016/S1389-1723(00)80033-X
DO - 10.1016/S1389-1723(00)80033-X
M3 - Article
C2 - 16232817
AN - SCOPUS:0034233142
SN - 1389-1723
VL - 90
SP - 52
EP - 56
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -