Cellular uptake study and cytotoxicity study of resveratrol-gold-peg-folate (RSVAU-PEG-FA) nanoparticles on hela human cervical cancer cell line

Dara A. Putri, Sutriyo Sutriyo, Fadlina C. Saputri

Research output: Contribution to journalArticle

Abstract

Objective: This study aimed to evaluate the effectivity of resveratrol-gold-PEG-folate (RSV-Au-PEG-FA) nanoparticles formulation in resveratrol (RSV) targeted delivery and cytotoxicity effect on HeLa human cervical cancer cell line. Methods: Gold nanoparticles (AuNP) were used as carriers and folic acid (FA) was used as active targeting moiety, using polyethylene glycol-bisamine (PEG-bis-amine) as linker. RSV-Au-PEG-FA nanoparticles were characterized by UV-Vis spectrophotometry, infrared spectroscopy, particle size analyzer (PSA), and transmission electron microscopy (TEM). Cellular uptake study was conducted by using fluorescence microscope. Cytotoxicity study was conducted by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: Cellular uptake study has shown that RSV-Au-PEG-FA nanoparticles are potential to be accumulated intracellularly in HeLa cells more than in Vero cells. Cytotoxicity study has shown RSV-Au-PEG-FA nanoparticles IC50 67.06±2.14 µM and RSV IC50 9.66±1.44 µM on HeLa cells Conclusion: RSV-Au-PEG-FA nanoparticles are potential to enhance RSV uptake by HeLa cells selectively.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalInternational Journal of Applied Pharmaceutics
Volume12
Issue number4
DOIs
Publication statusPublished - 1 Jul 2020

Keywords

  • Active targeting
  • Cellular uptake
  • Cytotoxicity
  • Folic acid
  • Gold nanoparticles
  • HeLa cells
  • Resveratrol

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