Cellular mRNA expressions of anti-oxidant factors in the blood of preeclamptic women

Masamitsu Nakamura, Akihiko Sekizawa, Yuditiya Purwosunu, Shiho Okazaki, Antonio Farina, Noroyono Wibowo, Hanako Shimizu, Takashi Okai

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Objective: To assess the alterations of mRNA expressions associated with oxidative stress in the cellular component of blood from pregnant women with pre-eclampsia. Methods: Peripheral blood samples were obtained from pregnant women with and without pre-eclampsia. Cellular RNA was subjected to a reverse transcription polymerase chain reaction (PCR) assay in order to examine the mRNA distribution among women with pre-eclampsia (n = 24) and control subjects (n = 24) during 35-41 weeks of gestation. The data were analyzed by non-parametric statistics. Results: Significant differences between the pre-eclampsia subjects and the controls were observed in the gene expressions associated with oxidative stress. Lower values in the pre-eclampsia group were found for heme oxygenase (HO)-1, HO-2, catalase and superoxide dismutase (SOD). The HO-1, HO-2 and the catalase levels significantly correlated with proteinuria, and the HO-2 level with systolic blood pressure. Conclusion: Significantly lower concentrations of HO-1, HO-2, SOD and catalase are found in the cellular component of blood from pre-eclamptic patients. The values correlate with the severity of pre-eclampsia. These findings indicate that enhanced oxidative stress and a decrease in the number of anti-oxidant enzymes may be associated with pre-eclampsia.

Original languageEnglish
Pages (from-to)691-696
Number of pages6
JournalPrenatal Diagnosis
Volume29
Issue number7
DOIs
Publication statusPublished - 1 Jul 2009

Keywords

  • Anti-oxidant enzymes
  • Cellular RNA in maternal blood
  • Fetal cells
  • Heme oxygenase-1
  • Nucleic acids and proteins
  • Pre-eclampsia
  • RNA

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