TY - JOUR
T1 - Cell viability inhibition effect of gallic acid and its synthetic derivative forms on primary cultured endometriosis cells
AU - Arleni, null
AU - Nareswari, Salma S.K.
AU - Sopiah, P. Popi
AU - Zoya, M. A.
AU - Arsianti, Ade
AU - Muharram, R.
N1 - Publisher Copyright:
© 2017 American Scientific Publishers All rights reserved.
PY - 2017/7
Y1 - 2017/7
N2 - Introduction: There is an urgency to find a treatment for endometriosis, preferably of herbal nature, since currently there is no cure for endometriosis that guarantees no recurrence. Since gallic acid and its synthetic derivatives have been known to reduce cell viability in several cancer cells, this study intended to investigate whether these substances also inhibit the viability of endometriosis cells. Method: Endometriosis cells were isolated and cultured from patients who had undergone laparoscopy surgery using enzymatic technique. After cells were 70–80% confluent, they were harvested and divided into 10,000 cells per well. Cells were given treatment of gallic acid and two of its synthetic derivatives forms-heptyl gallate and octyl gallate-for 48 hours; each with three dosage of 25.6 μg/ml, 51.2 μg/ml and 102.4 μg/ml. All interventions were made in a set of triplo, along with control and blank. MTS assay was used to observe the viability of the endometriosis cells. Results: After the data was rationalised with control, all three substances showed inhibition of endometriosis cells viability. The highest inhibition for all treatments was at 102.4 μg/ml, with inhibition percentage of 56.481%, 65.028%, and 56.839% for gallic acid, heptyl gallate, and octyl gallate, respectively. Conclusion: The present study suggests that gallic acid, heptyl gallate and octyl gallate, have an inhibition effect towards the viability of endometriosis cell.
AB - Introduction: There is an urgency to find a treatment for endometriosis, preferably of herbal nature, since currently there is no cure for endometriosis that guarantees no recurrence. Since gallic acid and its synthetic derivatives have been known to reduce cell viability in several cancer cells, this study intended to investigate whether these substances also inhibit the viability of endometriosis cells. Method: Endometriosis cells were isolated and cultured from patients who had undergone laparoscopy surgery using enzymatic technique. After cells were 70–80% confluent, they were harvested and divided into 10,000 cells per well. Cells were given treatment of gallic acid and two of its synthetic derivatives forms-heptyl gallate and octyl gallate-for 48 hours; each with three dosage of 25.6 μg/ml, 51.2 μg/ml and 102.4 μg/ml. All interventions were made in a set of triplo, along with control and blank. MTS assay was used to observe the viability of the endometriosis cells. Results: After the data was rationalised with control, all three substances showed inhibition of endometriosis cells viability. The highest inhibition for all treatments was at 102.4 μg/ml, with inhibition percentage of 56.481%, 65.028%, and 56.839% for gallic acid, heptyl gallate, and octyl gallate, respectively. Conclusion: The present study suggests that gallic acid, heptyl gallate and octyl gallate, have an inhibition effect towards the viability of endometriosis cell.
KW - Cell viability
KW - Endometriosis
KW - Gallic acid
KW - Heptyl gallate
KW - MTS assay
KW - Octyl gallate
UR - http://www.scopus.com/inward/record.url?scp=85030228258&partnerID=8YFLogxK
U2 - 10.1166/asl.2017.9371
DO - 10.1166/asl.2017.9371
M3 - Article
AN - SCOPUS:85030228258
SN - 1936-6612
VL - 23
SP - 6681
EP - 6684
JO - Advanced Science Letters
JF - Advanced Science Letters
IS - 7
ER -