Cell viability inhibition effect of gallic acid and its synthetic derivative forms on primary cultured endometriosis cells

Arleni, Salma S.K. Nareswari, P. Popi Sopiah, M. A. Zoya, Ade Arsianti, R. Muharram

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Abstract

Introduction: There is an urgency to find a treatment for endometriosis, preferably of herbal nature, since currently there is no cure for endometriosis that guarantees no recurrence. Since gallic acid and its synthetic derivatives have been known to reduce cell viability in several cancer cells, this study intended to investigate whether these substances also inhibit the viability of endometriosis cells. Method: Endometriosis cells were isolated and cultured from patients who had undergone laparoscopy surgery using enzymatic technique. After cells were 70–80% confluent, they were harvested and divided into 10,000 cells per well. Cells were given treatment of gallic acid and two of its synthetic derivatives forms-heptyl gallate and octyl gallate-for 48 hours; each with three dosage of 25.6 μg/ml, 51.2 μg/ml and 102.4 μg/ml. All interventions were made in a set of triplo, along with control and blank. MTS assay was used to observe the viability of the endometriosis cells. Results: After the data was rationalised with control, all three substances showed inhibition of endometriosis cells viability. The highest inhibition for all treatments was at 102.4 μg/ml, with inhibition percentage of 56.481%, 65.028%, and 56.839% for gallic acid, heptyl gallate, and octyl gallate, respectively. Conclusion: The present study suggests that gallic acid, heptyl gallate and octyl gallate, have an inhibition effect towards the viability of endometriosis cell.

Original languageEnglish
Pages (from-to)6681-6684
Number of pages4
JournalAdvanced Science Letters
Volume23
Issue number7
DOIs
Publication statusPublished - 1 Jul 2017

Keywords

  • Cell viability
  • Endometriosis
  • Gallic acid
  • Heptyl gallate
  • MTS assay
  • Octyl gallate

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