Cell pausing method for adipose tissue derived mesenchymal stem cells: Comparison of Petaka G3 and ordinary flask

Jeanne Adiwinata Pawitan, Isabella Kurnia Liem, Tera Dria Kispa, Evah Luviah, Fajar Mujadid, Novialdi

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Various methods of cell pausing were applied to prevent proliferation of cultured cells, which either required special medium, using complicated encapsulation method, or using a special flask (Petaka G3 flask). Therefore, this study aimed to develop an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT-MSCs) using ordinary basal medium and flask that yielded AT-MSCs, which were compliant with the requirements of International society for cell therapy (ISCT). Methods: We assessed cell numbers and viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs, which were cultured until 80% confluent and then placed at 4 ? or room temperature for three to seven days. We assessed those parameters between fully filled and sealed ordinary flasks compared to Petaka G3 flasks. Results: Cell numbers, viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs at room temperature were better than at 4 ?, and at room temperature, results of ordinary flasks were comparable to Petaka G3 flasks. Conclusion: We have developed an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT-MSCs), using ordinary basal medium and flask.

Original languageEnglish
JournalStem Cell Investigation
Volume7
DOIs
Publication statusPublished - 2020

Keywords

  • Adipose tissue
  • Cell pausing
  • Mesenchymal stem cells
  • Petaka g3 flask

Fingerprint

Dive into the research topics of 'Cell pausing method for adipose tissue derived mesenchymal stem cells: Comparison of Petaka G3 and ordinary flask'. Together they form a unique fingerprint.

Cite this