TY - JOUR
T1 - Cell pausing method for adipose tissue derived mesenchymal stem cells
T2 - Comparison of Petaka G3 and ordinary flask
AU - Pawitan, Jeanne Adiwinata
AU - Liem, Isabella Kurnia
AU - Kispa, Tera Dria
AU - Luviah, Evah
AU - Mujadid, Fajar
AU - Novialdi,
N1 - Funding Information:
Funding: This study was funded by a research grant from the
Publisher Copyright:
© Stem Cell Investigation. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Background: Various methods of cell pausing were applied to prevent proliferation of cultured cells, which either required special medium, using complicated encapsulation method, or using a special flask (Petaka G3 flask). Therefore, this study aimed to develop an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT-MSCs) using ordinary basal medium and flask that yielded AT-MSCs, which were compliant with the requirements of International society for cell therapy (ISCT). Methods: We assessed cell numbers and viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs, which were cultured until 80% confluent and then placed at 4 ? or room temperature for three to seven days. We assessed those parameters between fully filled and sealed ordinary flasks compared to Petaka G3 flasks. Results: Cell numbers, viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs at room temperature were better than at 4 ?, and at room temperature, results of ordinary flasks were comparable to Petaka G3 flasks. Conclusion: We have developed an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT-MSCs), using ordinary basal medium and flask.
AB - Background: Various methods of cell pausing were applied to prevent proliferation of cultured cells, which either required special medium, using complicated encapsulation method, or using a special flask (Petaka G3 flask). Therefore, this study aimed to develop an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT-MSCs) using ordinary basal medium and flask that yielded AT-MSCs, which were compliant with the requirements of International society for cell therapy (ISCT). Methods: We assessed cell numbers and viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs, which were cultured until 80% confluent and then placed at 4 ? or room temperature for three to seven days. We assessed those parameters between fully filled and sealed ordinary flasks compared to Petaka G3 flasks. Results: Cell numbers, viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs at room temperature were better than at 4 ?, and at room temperature, results of ordinary flasks were comparable to Petaka G3 flasks. Conclusion: We have developed an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT-MSCs), using ordinary basal medium and flask.
KW - Adipose tissue
KW - Cell pausing
KW - Mesenchymal stem cells
KW - Petaka g3 flask
UR - http://www.scopus.com/inward/record.url?scp=85111170904&partnerID=8YFLogxK
U2 - 10.21037/SCI.2019.12.01
DO - 10.21037/SCI.2019.12.01
M3 - Article
AN - SCOPUS:85111170904
SN - 2306-9759
VL - 7
JO - Stem Cell Investigation
JF - Stem Cell Investigation
ER -