TY - JOUR
T1 - Carrier RNA (cRNA) Enhances dsDNA Recovery Extracted from Small Volume Spent Embryo Culture Medium
AU - Sundari, Ayu Mulia
AU - Iffanolida, Pritta Ameilia
AU - Mutia, Kresna
AU - Muna, Naylah
AU - Mansyur, Eliza
AU - Hestiantoro, Andon
AU - Wiweko, Budi
AU - Fauzi, Muhammad
AU - Bowolaksono, Anom
N1 - Funding Information:
ACKNOWLEDGMENT This study is funded by the Directorate of Research and Community Service, University of Indonesia, and supported by an international publication grant: International Research Collaboration Grant (NKB-1944/UN2.R3.1/HKP.05.00/2019) of the year 2019.
Publisher Copyright:
© 2021. All Rights Reserved.
PY - 2021
Y1 - 2021
N2 - Embryo spent culture medium has been intensively investigated, considering its promising feature for non-invasive bioanalytical techniques in in-vitro fertilization (IVF). Despite, isolating DNA from such samples is quite challenging due to its small volume. Carrier RNA is reported to exhibit DNA retrieval effects and commonly employed in various limited biological samples, but there are no reports regarding its benefit on embryo media. Therefore, we aim to evaluate the competence of cRNA on isolated DNA from embryo medium and analyzed its optimal volume as there are also no records respecting its ideal volume to obtain decent outcomes. Results showed that cRNA significantly increases DNA amounts in the cRNA treated group (p<0.001), but the D-4/D-5 medium yielded similar (p=0.684). Pearson test demonstrated no correlation between cRNA volume vs. total retrieved DNA (r=0.760, p=0.80), and Whole Genome Amplification (WGA) was shown to increase DNA in the treated group (p=0.022), but not in the untreated group (p=0.128). Additionally, electrophoresis successfully resulting in a thick and thin band of TH01 locus signifies the cRNA competence. In conclusion, our study suggests that cRNA addition is essential in embryo medium extraction as it increases initial DNA that crucial for downstream application. However, the optimal volume could not be determined in the current study since the initial amount of DNA in the medium is unknown. Obtained findings are expected to be a new input for subsequent research on DNA extraction.
AB - Embryo spent culture medium has been intensively investigated, considering its promising feature for non-invasive bioanalytical techniques in in-vitro fertilization (IVF). Despite, isolating DNA from such samples is quite challenging due to its small volume. Carrier RNA is reported to exhibit DNA retrieval effects and commonly employed in various limited biological samples, but there are no reports regarding its benefit on embryo media. Therefore, we aim to evaluate the competence of cRNA on isolated DNA from embryo medium and analyzed its optimal volume as there are also no records respecting its ideal volume to obtain decent outcomes. Results showed that cRNA significantly increases DNA amounts in the cRNA treated group (p<0.001), but the D-4/D-5 medium yielded similar (p=0.684). Pearson test demonstrated no correlation between cRNA volume vs. total retrieved DNA (r=0.760, p=0.80), and Whole Genome Amplification (WGA) was shown to increase DNA in the treated group (p=0.022), but not in the untreated group (p=0.128). Additionally, electrophoresis successfully resulting in a thick and thin band of TH01 locus signifies the cRNA competence. In conclusion, our study suggests that cRNA addition is essential in embryo medium extraction as it increases initial DNA that crucial for downstream application. However, the optimal volume could not be determined in the current study since the initial amount of DNA in the medium is unknown. Obtained findings are expected to be a new input for subsequent research on DNA extraction.
KW - Assisted reproductive technology
KW - carrier RNA
KW - in-vitro fertilization
KW - spent embryo medium
UR - http://www.scopus.com/inward/record.url?scp=85110438610&partnerID=8YFLogxK
U2 - 10.18517/ijaseit.11.3.13591
DO - 10.18517/ijaseit.11.3.13591
M3 - Article
AN - SCOPUS:85110438610
SN - 2088-5334
VL - 11
SP - 1203
EP - 1208
JO - International Journal on Advanced Science, Engineering and Information Technology
JF - International Journal on Advanced Science, Engineering and Information Technology
IS - 3
ER -