Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue

Retno Wahyu Nurhayati; Normalina Sandora; Suwarti; Raisa Nauli; Zakiyah Khoiriyah; Aditya Wardhana

doi:10.1063/1.5139332

Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue

Retno Wahyu Nurhayati, Normalina Sandora, Suwarti, Raisa Nauli, Zakiyah Khoiriyah, Aditya Wardhana

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

The healing of skin wounds involves various factors including keratinocytes, fibroblasts, extracellular matrix, nervous and vascular components of the surrounding tissue. During wound healing, keratinocytes migrate from basal area to cover the lesion area. The important role of keratinocytes in skin regeneration triggers interests to isolate and expand the cells in vitro. This study was attempted to compare briefly enzymatic and non-enzymatic keratinocytes isolation from adult skin. Non-enzymatic based keratinocyte isolation through explant culture allowed minimal sample preparation, however, the process required longer preparation time in which the target cells typically appears after 2 weeks culturing period. Meanwhile, enzymatic-based keratinocyte isolation required more complex procedure with relatively shorter preparation time. It is likely that explant method produced higher purity of keratinocyte stem cells as compared with direct digestion method, as analyzed from cytokeratin 14 expression.

Original language	English
Title of host publication	4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019
Editors	Kenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735419445
DOIs	https://doi.org/10.1063/1.5139332
Publication status	Published - 10 Dec 2019
Event	4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia Duration: 22 Jul 2019 → 24 Jul 2019

Publication series

Name	AIP Conference Proceedings
Volume	2193
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Country/Territory	Indonesia
City	Padang, West Sumatera
Period	22/07/19 → 24/07/19

Keywords

cytokeratin
enzymatic digestion
keratinocyte
skin
tissue

Access to Document

10.1063/1.5139332

Cite this

Nurhayati, R. W., Sandora, N., Suwarti, Nauli, R., Khoiriyah, Z., & Wardhana, A. (2019). Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue. In K. Lischer, T. Abuzairi, S. F. Rahman, & M. Gozan (Eds.), 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019 Article 020012 (AIP Conference Proceedings; Vol. 2193). American Institute of Physics Inc.. https://doi.org/10.1063/1.5139332

Nurhayati, Retno Wahyu ; Sandora, Normalina ; Suwarti et al. / Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue. 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. editor / Kenny Lischer ; Tomy Abuzairi ; Siti Fauziyah Rahman ; Misri Gozan. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

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title = "Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue",

abstract = "The healing of skin wounds involves various factors including keratinocytes, fibroblasts, extracellular matrix, nervous and vascular components of the surrounding tissue. During wound healing, keratinocytes migrate from basal area to cover the lesion area. The important role of keratinocytes in skin regeneration triggers interests to isolate and expand the cells in vitro. This study was attempted to compare briefly enzymatic and non-enzymatic keratinocytes isolation from adult skin. Non-enzymatic based keratinocyte isolation through explant culture allowed minimal sample preparation, however, the process required longer preparation time in which the target cells typically appears after 2 weeks culturing period. Meanwhile, enzymatic-based keratinocyte isolation required more complex procedure with relatively shorter preparation time. It is likely that explant method produced higher purity of keratinocyte stem cells as compared with direct digestion method, as analyzed from cytokeratin 14 expression.",

keywords = "cytokeratin, enzymatic digestion, keratinocyte, skin, tissue",

author = "Nurhayati, {Retno Wahyu} and Normalina Sandora and Suwarti and Raisa Nauli and Zakiyah Khoiriyah and Aditya Wardhana",

note = "Funding Information: This research is funded by Penelitian Terapan Unggulan Perguruan Tinggi (PTUPT) Universitas Riau grant from Indonesian Ministry of Research and Technology and Higher Education lead by Dr Normalina Sandora (Contract No: 792/UN.19.5.1.3/PT.01.03/2019). Publisher Copyright: {\textcopyright} 2019 Author(s).; 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 ; Conference date: 22-07-2019 Through 24-07-2019",

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publisher = "American Institute of Physics Inc.",

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Nurhayati, RW, Sandora, N, Suwarti, Nauli, R, Khoiriyah, Z & Wardhana, A 2019, Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue. in K Lischer, T Abuzairi, SF Rahman & M Gozan (eds), 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019., 020012, AIP Conference Proceedings, vol. 2193, American Institute of Physics Inc., 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019, Padang, West Sumatera, Indonesia, 22/07/19. https://doi.org/10.1063/1.5139332

Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue. / Nurhayati, Retno Wahyu; Sandora, Normalina; Suwarti et al.
4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. ed. / Kenny Lischer; Tomy Abuzairi; Siti Fauziyah Rahman; Misri Gozan. American Institute of Physics Inc., 2019. 020012 (AIP Conference Proceedings; Vol. 2193).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Nurhayati, Retno Wahyu

AU - Sandora, Normalina

AU - Suwarti,

AU - Nauli, Raisa

AU - Khoiriyah, Zakiyah

AU - Wardhana, Aditya

N1 - Funding Information: This research is funded by Penelitian Terapan Unggulan Perguruan Tinggi (PTUPT) Universitas Riau grant from Indonesian Ministry of Research and Technology and Higher Education lead by Dr Normalina Sandora (Contract No: 792/UN.19.5.1.3/PT.01.03/2019). Publisher Copyright: © 2019 Author(s).

PY - 2019/12/10

Y1 - 2019/12/10

N2 - The healing of skin wounds involves various factors including keratinocytes, fibroblasts, extracellular matrix, nervous and vascular components of the surrounding tissue. During wound healing, keratinocytes migrate from basal area to cover the lesion area. The important role of keratinocytes in skin regeneration triggers interests to isolate and expand the cells in vitro. This study was attempted to compare briefly enzymatic and non-enzymatic keratinocytes isolation from adult skin. Non-enzymatic based keratinocyte isolation through explant culture allowed minimal sample preparation, however, the process required longer preparation time in which the target cells typically appears after 2 weeks culturing period. Meanwhile, enzymatic-based keratinocyte isolation required more complex procedure with relatively shorter preparation time. It is likely that explant method produced higher purity of keratinocyte stem cells as compared with direct digestion method, as analyzed from cytokeratin 14 expression.

AB - The healing of skin wounds involves various factors including keratinocytes, fibroblasts, extracellular matrix, nervous and vascular components of the surrounding tissue. During wound healing, keratinocytes migrate from basal area to cover the lesion area. The important role of keratinocytes in skin regeneration triggers interests to isolate and expand the cells in vitro. This study was attempted to compare briefly enzymatic and non-enzymatic keratinocytes isolation from adult skin. Non-enzymatic based keratinocyte isolation through explant culture allowed minimal sample preparation, however, the process required longer preparation time in which the target cells typically appears after 2 weeks culturing period. Meanwhile, enzymatic-based keratinocyte isolation required more complex procedure with relatively shorter preparation time. It is likely that explant method produced higher purity of keratinocyte stem cells as compared with direct digestion method, as analyzed from cytokeratin 14 expression.

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BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices

A2 - Lischer, Kenny

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Nurhayati RW, Sandora N, Suwarti, Nauli R, Khoiriyah Z, Wardhana A. Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue. In Lischer K, Abuzairi T, Rahman SF, Gozan M, editors, 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. American Institute of Physics Inc. 2019. 020012. (AIP Conference Proceedings). doi: 10.1063/1.5139332

Brief comparative study on the isolation and culture methods of human keratinocyte from skin tissue

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