Biosurfactant saponin from leaf extract of Morinda citrifolia L. as emulsifier to improve β-carotene solubility in microemulsion

T. C.D. Tania, Y. N. Deastri, Tresye Utari, Yoki Yulizar, D. O.B. Apriandanu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Leaf extract of Morinda citrofilia L. contains several types of secondary metabolites; one of them is biosurfactant saponin which can be used as emulsifier in microemulsion formation. Saponin extraction was performed with maceration technique, identified by phytochemical test and characterized using FTIR and UV-Vis spectrophotometer. β-carotene is a natural colorant which frequently used in beverages but it is vulnerable to temperatures and lights. Palm oil was used as oil phase. β-carotene solubilization in microemulsion increases the stability. Microemulsion formation was performed by varying the surfactant, co-surfactant and oil phase. Microemulsion stability was observed using turbidimeter, microscope, and particle size analyzer (PSA). β-carotene solubility and stability in microemulsion system were observed using UV-Vis spectrophotometer. Microemulsion particle size was confirmed at 9.65 nm. The stabilized oil in water microemulsion was obtained with ratio of surfactant and co-surfactant (Sm) at 8:2, and ratio of Sm and oil at 14:1. β-carotene optimum solubility in microemulsion system was obtained at 2.00 mg/mL.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Morinda citrifolia L. emulsifier
  • Saponin
  • microemulsion
  • β-carotene

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    Tania, T. C. D., Deastri, Y. N., Utari, T., Yulizar, Y., & Apriandanu, D. O. B. (2018). Biosurfactant saponin from leaf extract of Morinda citrifolia L. as emulsifier to improve β-carotene solubility in microemulsion. In R. Yuniati, T. Mart, I. T. Anggraningrum, D. Triyono, & K. A. Sugeng (Eds.), Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 [020103] (AIP Conference Proceedings; Vol. 2023). American Institute of Physics Inc.. https://doi.org/10.1063/1.5064100